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human β2-microglobulin  (Millipore)


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    Millipore human β2-microglobulin
    Human β2 Microglobulin, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+%CE%B22-microglobulin/human+%CE%B22+microglobulin/us12331097-1543-24-26
    Average 90 stars, based on 1 article reviews
    human β2-microglobulin - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: Identification of an HLA-A2-restricted CD147 epitope that can induce specific CTL cytotoxicity against drug resistant MCF-7/Adr cells
    Article Snippet: Briefly, T2 cells (1×10 6 /group) were incubated in the presence of 20 μg/ml peptide in AIMV medium (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 5 μg/ml human β2-microglobulin (Sigma-Aldrich, Spain) at 37°C in 5% CO 2 for 18 h. T2 cells were washed twice with PBS to remove unbound peptide and resuspended in PBS containing 2% FBS.

    Article Title: Prediction and identification of HLA-A*0201-restricted epitopes from cancer testis antigen CT23.
    Article Snippet: In this experiment, T2 cell binding experiments were performed on CT23 candidate peptides predicted above, and the experimental steps were carried out with reference to literatures.24,25 T2 cells (1 × 106/mL) were incubated in the presence of 50 μg/ mL peptide in RPMI-1640 medium supplemented with 3 μg/mL human β2-microglobulin (Sigma, USA) at 37°C in 5% CO2 for 18 h, and the following tests were performed, respectively.

    Article Title: Novel Survivin Peptides Screened With Computer Algorithm Induce Cytotoxic T Lymphocytes With Higher Cytotoxic Efficiency to Cancer Cells
    Article Snippet: A number of 1 × 10 6 cells were added into 1 ml AIM-V medium (Life Technologies, Carlsbad, CA, United States) and incubated with human β2-microglobulin (EMD Biosciences, San Diego, CA, United States) at 3 μg/ml and peptide including either positive control peptide (POL476, ILKEPVHGV) or each SV 95 peptide (from SV 95–1 to SV 95–10 ) at various final concentrations of 10, 0.1, and 0.01 μM at 37°C for 21 h. One day later, T2 cells were incubated with anti-HLA-A2.1 (BB7.2) mAb at a saturating concentration for 30 min at 4°C.

    Article Title: Identification of a novel conserved HLA-A*0201-restricted epitope from the spike protein of SARS-CoV
    Article Snippet: Briefly, T2 cells were incubated with 20 μg/ml candidate peptides and 3 μg/ml human β2-microglobulin (Sigma, St Louis, MO) in serum-free RPMI1640 for 18 hours at 37°C in a 5% CO 2 atmosphere.

    Article Title: T cell receptor constructs and uses thereof
    Article Snippet: For exogenous peptide pulsing, 1×106 T2, 293T or A375 cells were incubated at 37° C. and 5% CO2 for 2 h with 10 pg/mL human β2-microglobulin (Calbiochem) and titrating amounts, ranging from 1×10−5 M to 1×10−12 M of the RAS or GATA3 peptides, T2 cells pulsed with 10−5M influenza peptide GIL (influenza matrix protein58-66 GILGFVTL, Metabion) served as negative control.

    Article Title: Unique roles of co-receptor-bound LCK in helper and cytotoxic T cells
    Article Snippet: Afterward, the sponges were placed in 2 ml of RPMI supplemented with 10% FBS, 50 μM β-mercaptoethanol, 2 mM l -glutamine, 100 U ml –1 penicillin, 100 mg ml –1 streptomycin, 50 mg ml –1 gentamicin and 5 μg ml –1 human β2-microglobulin (Sigma-Aldrich, 475823-M) in a six-well plate.

    Article Title: Identification and characterization of HLA-A24 specific XBP1, CD138 (Syndecan-1), and CS1 (SLAMF7) peptides inducing antigens-specific memory cytotoxic T lymphocytes targeting multiple myeloma
    Article Snippet: The cells were pulsed with various concentrations (0 – 200 μg/ml) of respective unspliced XBP1, spliced XBP1, CD138 or CS1 peptide, or with a control HIV-1 envelope protein gp41 583-591 peptide (50 μg/ml) plus human β2-microglobulin (3 μg/ml) (Sigma), and then incubated at 26~28°C in 5% CO 2 humidified air.

    Article Title: High affinity T cell receptor and use thereof
    Article Snippet: Peptide Loading of T2 Cells, PBMC and Tumor Cells For exogenous peptide pulsing, 1×106 T2 cells were incubated at 37° C. and 5% CO2 for 2 h with 10 μg/ml human β2-microglobulin (Calbiochem) and titrating amounts, ranging from 10−5 M to 10−11 M, of the tyrosinase peptide YMD (tyrosinase369-377 YMDGTMSQV, SEQ ID NO: 9, Metabion).

    Negative Control:

    Article Title: Identification of an HLA-A2-restricted CD147 epitope that can induce specific CTL cytotoxicity against drug resistant MCF-7/Adr cells
    Article Snippet: Briefly, T2 cells (1×10 6 /group) were incubated in the presence of 20 μg/ml peptide in AIMV medium (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 5 μg/ml human β2-microglobulin (Sigma-Aldrich, Spain) at 37°C in 5% CO 2 for 18 h. T2 cells were washed twice with PBS to remove unbound peptide and resuspended in PBS containing 2% FBS.

    Article Title: Prediction and identification of HLA-A*0201-restricted epitopes from cancer testis antigen CT23.
    Article Snippet: In this experiment, T2 cell binding experiments were performed on CT23 candidate peptides predicted above, and the experimental steps were carried out with reference to literatures.24,25 T2 cells (1 × 106/mL) were incubated in the presence of 50 μg/ mL peptide in RPMI-1640 medium supplemented with 3 μg/mL human β2-microglobulin (Sigma, USA) at 37°C in 5% CO2 for 18 h, and the following tests were performed, respectively.

    Article Title: Novel Survivin Peptides Screened With Computer Algorithm Induce Cytotoxic T Lymphocytes With Higher Cytotoxic Efficiency to Cancer Cells
    Article Snippet: A number of 1 × 10 6 cells were added into 1 ml AIM-V medium (Life Technologies, Carlsbad, CA, United States) and incubated with human β2-microglobulin (EMD Biosciences, San Diego, CA, United States) at 3 μg/ml and peptide including either positive control peptide (POL476, ILKEPVHGV) or each SV 95 peptide (from SV 95–1 to SV 95–10 ) at various final concentrations of 10, 0.1, and 0.01 μM at 37°C for 21 h. One day later, T2 cells were incubated with anti-HLA-A2.1 (BB7.2) mAb at a saturating concentration for 30 min at 4°C.

    Article Title: Identification of a novel conserved HLA-A*0201-restricted epitope from the spike protein of SARS-CoV
    Article Snippet: Briefly, T2 cells were incubated with 20 μg/ml candidate peptides and 3 μg/ml human β2-microglobulin (Sigma, St Louis, MO) in serum-free RPMI1640 for 18 hours at 37°C in a 5% CO 2 atmosphere.

    Article Title: T cell receptor constructs and uses thereof
    Article Snippet: For exogenous peptide pulsing, 1×106 T2, 293T or A375 cells were incubated at 37° C. and 5% CO2 for 2 h with 10 pg/mL human β2-microglobulin (Calbiochem) and titrating amounts, ranging from 1×10−5 M to 1×10−12 M of the RAS or GATA3 peptides, T2 cells pulsed with 10−5M influenza peptide GIL (influenza matrix protein58-66 GILGFVTL, Metabion) served as negative control.

    Article Title: Unique roles of co-receptor-bound LCK in helper and cytotoxic T cells
    Article Snippet: Afterward, the sponges were placed in 2 ml of RPMI supplemented with 10% FBS, 50 μM β-mercaptoethanol, 2 mM l -glutamine, 100 U ml –1 penicillin, 100 mg ml –1 streptomycin, 50 mg ml –1 gentamicin and 5 μg ml –1 human β2-microglobulin (Sigma-Aldrich, 475823-M) in a six-well plate.

    Article Title: Identification and characterization of HLA-A24 specific XBP1, CD138 (Syndecan-1), and CS1 (SLAMF7) peptides inducing antigens-specific memory cytotoxic T lymphocytes targeting multiple myeloma
    Article Snippet: The cells were pulsed with various concentrations (0 – 200 μg/ml) of respective unspliced XBP1, spliced XBP1, CD138 or CS1 peptide, or with a control HIV-1 envelope protein gp41 583-591 peptide (50 μg/ml) plus human β2-microglobulin (3 μg/ml) (Sigma), and then incubated at 26~28°C in 5% CO 2 humidified air.

    Article Title: High affinity T cell receptor and use thereof
    Article Snippet: Peptide Loading of T2 Cells, PBMC and Tumor Cells For exogenous peptide pulsing, 1×106 T2 cells were incubated at 37° C. and 5% CO2 for 2 h with 10 μg/ml human β2-microglobulin (Calbiochem) and titrating amounts, ranging from 10−5 M to 10−11 M, of the tyrosinase peptide YMD (tyrosinase369-377 YMDGTMSQV, SEQ ID NO: 9, Metabion).

    Binding Assay:

    Article Title: Identification of an HLA-A2-restricted CD147 epitope that can induce specific CTL cytotoxicity against drug resistant MCF-7/Adr cells
    Article Snippet: Briefly, T2 cells (1×10 6 /group) were incubated in the presence of 20 μg/ml peptide in AIMV medium (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 5 μg/ml human β2-microglobulin (Sigma-Aldrich, Spain) at 37°C in 5% CO 2 for 18 h. T2 cells were washed twice with PBS to remove unbound peptide and resuspended in PBS containing 2% FBS.

    Article Title: Prediction and identification of HLA-A*0201-restricted epitopes from cancer testis antigen CT23.
    Article Snippet: In this experiment, T2 cell binding experiments were performed on CT23 candidate peptides predicted above, and the experimental steps were carried out with reference to literatures.24,25 T2 cells (1 × 106/mL) were incubated in the presence of 50 μg/ mL peptide in RPMI-1640 medium supplemented with 3 μg/mL human β2-microglobulin (Sigma, USA) at 37°C in 5% CO2 for 18 h, and the following tests were performed, respectively.

    Article Title: Novel Survivin Peptides Screened With Computer Algorithm Induce Cytotoxic T Lymphocytes With Higher Cytotoxic Efficiency to Cancer Cells
    Article Snippet: A number of 1 × 10 6 cells were added into 1 ml AIM-V medium (Life Technologies, Carlsbad, CA, United States) and incubated with human β2-microglobulin (EMD Biosciences, San Diego, CA, United States) at 3 μg/ml and peptide including either positive control peptide (POL476, ILKEPVHGV) or each SV 95 peptide (from SV 95–1 to SV 95–10 ) at various final concentrations of 10, 0.1, and 0.01 μM at 37°C for 21 h. One day later, T2 cells were incubated with anti-HLA-A2.1 (BB7.2) mAb at a saturating concentration for 30 min at 4°C.

    Article Title: Identification of a novel conserved HLA-A*0201-restricted epitope from the spike protein of SARS-CoV
    Article Snippet: Briefly, T2 cells were incubated with 20 μg/ml candidate peptides and 3 μg/ml human β2-microglobulin (Sigma, St Louis, MO) in serum-free RPMI1640 for 18 hours at 37°C in a 5% CO 2 atmosphere.

    Article Title: T cell receptor constructs and uses thereof
    Article Snippet: For exogenous peptide pulsing, 1×106 T2, 293T or A375 cells were incubated at 37° C. and 5% CO2 for 2 h with 10 pg/mL human β2-microglobulin (Calbiochem) and titrating amounts, ranging from 1×10−5 M to 1×10−12 M of the RAS or GATA3 peptides, T2 cells pulsed with 10−5M influenza peptide GIL (influenza matrix protein58-66 GILGFVTL, Metabion) served as negative control.

    Article Title: Unique roles of co-receptor-bound LCK in helper and cytotoxic T cells
    Article Snippet: Afterward, the sponges were placed in 2 ml of RPMI supplemented with 10% FBS, 50 μM β-mercaptoethanol, 2 mM l -glutamine, 100 U ml –1 penicillin, 100 mg ml –1 streptomycin, 50 mg ml –1 gentamicin and 5 μg ml –1 human β2-microglobulin (Sigma-Aldrich, 475823-M) in a six-well plate.

    Article Title: Identification and characterization of HLA-A24 specific XBP1, CD138 (Syndecan-1), and CS1 (SLAMF7) peptides inducing antigens-specific memory cytotoxic T lymphocytes targeting multiple myeloma
    Article Snippet: The cells were pulsed with various concentrations (0 – 200 μg/ml) of respective unspliced XBP1, spliced XBP1, CD138 or CS1 peptide, or with a control HIV-1 envelope protein gp41 583-591 peptide (50 μg/ml) plus human β2-microglobulin (3 μg/ml) (Sigma), and then incubated at 26~28°C in 5% CO 2 humidified air.

    Article Title: High affinity T cell receptor and use thereof
    Article Snippet: Peptide Loading of T2 Cells, PBMC and Tumor Cells For exogenous peptide pulsing, 1×106 T2 cells were incubated at 37° C. and 5% CO2 for 2 h with 10 μg/ml human β2-microglobulin (Calbiochem) and titrating amounts, ranging from 10−5 M to 10−11 M, of the tyrosinase peptide YMD (tyrosinase369-377 YMDGTMSQV, SEQ ID NO: 9, Metabion).

    Positive Control:

    Article Title: Identification of an HLA-A2-restricted CD147 epitope that can induce specific CTL cytotoxicity against drug resistant MCF-7/Adr cells
    Article Snippet: Briefly, T2 cells (1×10 6 /group) were incubated in the presence of 20 μg/ml peptide in AIMV medium (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 5 μg/ml human β2-microglobulin (Sigma-Aldrich, Spain) at 37°C in 5% CO 2 for 18 h. T2 cells were washed twice with PBS to remove unbound peptide and resuspended in PBS containing 2% FBS.

    Article Title: Prediction and identification of HLA-A*0201-restricted epitopes from cancer testis antigen CT23.
    Article Snippet: In this experiment, T2 cell binding experiments were performed on CT23 candidate peptides predicted above, and the experimental steps were carried out with reference to literatures.24,25 T2 cells (1 × 106/mL) were incubated in the presence of 50 μg/ mL peptide in RPMI-1640 medium supplemented with 3 μg/mL human β2-microglobulin (Sigma, USA) at 37°C in 5% CO2 for 18 h, and the following tests were performed, respectively.

    Article Title: Novel Survivin Peptides Screened With Computer Algorithm Induce Cytotoxic T Lymphocytes With Higher Cytotoxic Efficiency to Cancer Cells
    Article Snippet: A number of 1 × 10 6 cells were added into 1 ml AIM-V medium (Life Technologies, Carlsbad, CA, United States) and incubated with human β2-microglobulin (EMD Biosciences, San Diego, CA, United States) at 3 μg/ml and peptide including either positive control peptide (POL476, ILKEPVHGV) or each SV 95 peptide (from SV 95–1 to SV 95–10 ) at various final concentrations of 10, 0.1, and 0.01 μM at 37°C for 21 h. One day later, T2 cells were incubated with anti-HLA-A2.1 (BB7.2) mAb at a saturating concentration for 30 min at 4°C.

    Article Title: Identification of a novel conserved HLA-A*0201-restricted epitope from the spike protein of SARS-CoV
    Article Snippet: Briefly, T2 cells were incubated with 20 μg/ml candidate peptides and 3 μg/ml human β2-microglobulin (Sigma, St Louis, MO) in serum-free RPMI1640 for 18 hours at 37°C in a 5% CO 2 atmosphere.

    Article Title: T cell receptor constructs and uses thereof
    Article Snippet: For exogenous peptide pulsing, 1×106 T2, 293T or A375 cells were incubated at 37° C. and 5% CO2 for 2 h with 10 pg/mL human β2-microglobulin (Calbiochem) and titrating amounts, ranging from 1×10−5 M to 1×10−12 M of the RAS or GATA3 peptides, T2 cells pulsed with 10−5M influenza peptide GIL (influenza matrix protein58-66 GILGFVTL, Metabion) served as negative control.

    Article Title: Unique roles of co-receptor-bound LCK in helper and cytotoxic T cells
    Article Snippet: Afterward, the sponges were placed in 2 ml of RPMI supplemented with 10% FBS, 50 μM β-mercaptoethanol, 2 mM l -glutamine, 100 U ml –1 penicillin, 100 mg ml –1 streptomycin, 50 mg ml –1 gentamicin and 5 μg ml –1 human β2-microglobulin (Sigma-Aldrich, 475823-M) in a six-well plate.

    Article Title: Identification and characterization of HLA-A24 specific XBP1, CD138 (Syndecan-1), and CS1 (SLAMF7) peptides inducing antigens-specific memory cytotoxic T lymphocytes targeting multiple myeloma
    Article Snippet: The cells were pulsed with various concentrations (0 – 200 μg/ml) of respective unspliced XBP1, spliced XBP1, CD138 or CS1 peptide, or with a control HIV-1 envelope protein gp41 583-591 peptide (50 μg/ml) plus human β2-microglobulin (3 μg/ml) (Sigma), and then incubated at 26~28°C in 5% CO 2 humidified air.

    Article Title: High affinity T cell receptor and use thereof
    Article Snippet: Peptide Loading of T2 Cells, PBMC and Tumor Cells For exogenous peptide pulsing, 1×106 T2 cells were incubated at 37° C. and 5% CO2 for 2 h with 10 μg/ml human β2-microglobulin (Calbiochem) and titrating amounts, ranging from 10−5 M to 10−11 M, of the tyrosinase peptide YMD (tyrosinase369-377 YMDGTMSQV, SEQ ID NO: 9, Metabion).

    Concentration Assay:

    Article Title: Identification of an HLA-A2-restricted CD147 epitope that can induce specific CTL cytotoxicity against drug resistant MCF-7/Adr cells
    Article Snippet: Briefly, T2 cells (1×10 6 /group) were incubated in the presence of 20 μg/ml peptide in AIMV medium (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 5 μg/ml human β2-microglobulin (Sigma-Aldrich, Spain) at 37°C in 5% CO 2 for 18 h. T2 cells were washed twice with PBS to remove unbound peptide and resuspended in PBS containing 2% FBS.

    Article Title: Prediction and identification of HLA-A*0201-restricted epitopes from cancer testis antigen CT23.
    Article Snippet: In this experiment, T2 cell binding experiments were performed on CT23 candidate peptides predicted above, and the experimental steps were carried out with reference to literatures.24,25 T2 cells (1 × 106/mL) were incubated in the presence of 50 μg/ mL peptide in RPMI-1640 medium supplemented with 3 μg/mL human β2-microglobulin (Sigma, USA) at 37°C in 5% CO2 for 18 h, and the following tests were performed, respectively.

    Article Title: Novel Survivin Peptides Screened With Computer Algorithm Induce Cytotoxic T Lymphocytes With Higher Cytotoxic Efficiency to Cancer Cells
    Article Snippet: A number of 1 × 10 6 cells were added into 1 ml AIM-V medium (Life Technologies, Carlsbad, CA, United States) and incubated with human β2-microglobulin (EMD Biosciences, San Diego, CA, United States) at 3 μg/ml and peptide including either positive control peptide (POL476, ILKEPVHGV) or each SV 95 peptide (from SV 95–1 to SV 95–10 ) at various final concentrations of 10, 0.1, and 0.01 μM at 37°C for 21 h. One day later, T2 cells were incubated with anti-HLA-A2.1 (BB7.2) mAb at a saturating concentration for 30 min at 4°C.

    Article Title: Identification of a novel conserved HLA-A*0201-restricted epitope from the spike protein of SARS-CoV
    Article Snippet: Briefly, T2 cells were incubated with 20 μg/ml candidate peptides and 3 μg/ml human β2-microglobulin (Sigma, St Louis, MO) in serum-free RPMI1640 for 18 hours at 37°C in a 5% CO 2 atmosphere.

    Article Title: T cell receptor constructs and uses thereof
    Article Snippet: For exogenous peptide pulsing, 1×106 T2, 293T or A375 cells were incubated at 37° C. and 5% CO2 for 2 h with 10 pg/mL human β2-microglobulin (Calbiochem) and titrating amounts, ranging from 1×10−5 M to 1×10−12 M of the RAS or GATA3 peptides, T2 cells pulsed with 10−5M influenza peptide GIL (influenza matrix protein58-66 GILGFVTL, Metabion) served as negative control.

    Article Title: Unique roles of co-receptor-bound LCK in helper and cytotoxic T cells
    Article Snippet: Afterward, the sponges were placed in 2 ml of RPMI supplemented with 10% FBS, 50 μM β-mercaptoethanol, 2 mM l -glutamine, 100 U ml –1 penicillin, 100 mg ml –1 streptomycin, 50 mg ml –1 gentamicin and 5 μg ml –1 human β2-microglobulin (Sigma-Aldrich, 475823-M) in a six-well plate.

    Article Title: Identification and characterization of HLA-A24 specific XBP1, CD138 (Syndecan-1), and CS1 (SLAMF7) peptides inducing antigens-specific memory cytotoxic T lymphocytes targeting multiple myeloma
    Article Snippet: The cells were pulsed with various concentrations (0 – 200 μg/ml) of respective unspliced XBP1, spliced XBP1, CD138 or CS1 peptide, or with a control HIV-1 envelope protein gp41 583-591 peptide (50 μg/ml) plus human β2-microglobulin (3 μg/ml) (Sigma), and then incubated at 26~28°C in 5% CO 2 humidified air.

    Article Title: High affinity T cell receptor and use thereof
    Article Snippet: Peptide Loading of T2 Cells, PBMC and Tumor Cells For exogenous peptide pulsing, 1×106 T2 cells were incubated at 37° C. and 5% CO2 for 2 h with 10 μg/ml human β2-microglobulin (Calbiochem) and titrating amounts, ranging from 10−5 M to 10−11 M, of the tyrosinase peptide YMD (tyrosinase369-377 YMDGTMSQV, SEQ ID NO: 9, Metabion).



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    93/100 stars
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    β2 microglobulin β2m plasmid  (OriGene)
    94
    OriGene β2 microglobulin β2m plasmid
    a – c A transcriptome analysis of mature adipocytes isolated from EpiWAT of C57BL/6J mice on 16-week NCD and HFD. a Network diagram illustrating the interaction among the top 10 immune-related pathways enriched by KEGG analysis of DEGs. The table showing the degree of interaction for each pathway, as assessed by the cumulative interaction scores of the genes within the pathway. b GSEA of DEGs showing two <t>B2M-associated</t> pathways in adipocytes. c Network diagram illustrating the interaction among genes in antigen processing and presentation pathway (blue) and iron uptake and transport pathway (green), with B2M (red) at the intersection. Relative B2m mRNA levels ( d ), protein expressions ( e ) and relative quantification ( f ) in EpiWAT, SVF and purified mature adipocyte fraction from EpiWAT of 16-week NCD- or HFD-fed mice ( n = 3-8). g Representative fluorescence images of B2M (green) and DAPI (blue) staining in EpiWAT sections of NCD-fed (upper) and HFD-fed (below) mice. The right panel quantifies the area-normalized B2M MFI (n = 3). Scale bar = 50 μm. Relative B2m mRNA levels ( h ), protein expressions ( i ) and relative quantification ( j ) in 3T3-L1 adipocytes treated with PA at the indicated dose for 24 h ( n = 4-5). The data are representative as the mean ± standard deviation (SD), with “n” representing the number of biological replicates per experimental group. Significant in d , f , and g was calculated using a two-tailed Student’s t test. Significant in h and j was calculated using one-way ANOVA followed by Tukey’s HSD post hoc test for multiple comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001. B2M β2-microglobulin, DEGs differentially expressed genes, EpiWAT epididymal adipose tissue, HFD high-fat diet, MFI mean fluorescence intensity, NCD normal chow diet, PA palmitic acid, SVF stromal vascular fraction
    β2 Microglobulin β2m Plasmid, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+%CE%B22-microglobulin/beta+2+Microglobulin+(B2M)+(NM_004048)+Human+3'+UTR+Clone/pm41016418-60-4-8
    Average 94 stars, based on 1 article reviews
    β2 microglobulin β2m plasmid - by Bioz Stars, 2026-10
    94/100 stars
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    b2m based stimulation  (Miltenyi Biotec)
    94
    Miltenyi Biotec b2m based stimulation
    a – c A transcriptome analysis of mature adipocytes isolated from EpiWAT of C57BL/6J mice on 16-week NCD and HFD. a Network diagram illustrating the interaction among the top 10 immune-related pathways enriched by KEGG analysis of DEGs. The table showing the degree of interaction for each pathway, as assessed by the cumulative interaction scores of the genes within the pathway. b GSEA of DEGs showing two <t>B2M-associated</t> pathways in adipocytes. c Network diagram illustrating the interaction among genes in antigen processing and presentation pathway (blue) and iron uptake and transport pathway (green), with B2M (red) at the intersection. Relative B2m mRNA levels ( d ), protein expressions ( e ) and relative quantification ( f ) in EpiWAT, SVF and purified mature adipocyte fraction from EpiWAT of 16-week NCD- or HFD-fed mice ( n = 3-8). g Representative fluorescence images of B2M (green) and DAPI (blue) staining in EpiWAT sections of NCD-fed (upper) and HFD-fed (below) mice. The right panel quantifies the area-normalized B2M MFI (n = 3). Scale bar = 50 μm. Relative B2m mRNA levels ( h ), protein expressions ( i ) and relative quantification ( j ) in 3T3-L1 adipocytes treated with PA at the indicated dose for 24 h ( n = 4-5). The data are representative as the mean ± standard deviation (SD), with “n” representing the number of biological replicates per experimental group. Significant in d , f , and g was calculated using a two-tailed Student’s t test. Significant in h and j was calculated using one-way ANOVA followed by Tukey’s HSD post hoc test for multiple comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001. B2M β2-microglobulin, DEGs differentially expressed genes, EpiWAT epididymal adipose tissue, HFD high-fat diet, MFI mean fluorescence intensity, NCD normal chow diet, PA palmitic acid, SVF stromal vascular fraction
    B2m Based Stimulation, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+%CE%B22-microglobulin/%CE%B22+Microglobulin+Antibody%2C+anti-human%2C+PE%2C+REAdye_lease/bio_rxiv__2025__08__06__668737-153-1-37
    Average 94 stars, based on 1 article reviews
    b2m based stimulation - by Bioz Stars, 2026-10
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    b2m  (Miltenyi Biotec)
    94
    Miltenyi Biotec b2m
    a – c A transcriptome analysis of mature adipocytes isolated from EpiWAT of C57BL/6J mice on 16-week NCD and HFD. a Network diagram illustrating the interaction among the top 10 immune-related pathways enriched by KEGG analysis of DEGs. The table showing the degree of interaction for each pathway, as assessed by the cumulative interaction scores of the genes within the pathway. b GSEA of DEGs showing two <t>B2M-associated</t> pathways in adipocytes. c Network diagram illustrating the interaction among genes in antigen processing and presentation pathway (blue) and iron uptake and transport pathway (green), with B2M (red) at the intersection. Relative B2m mRNA levels ( d ), protein expressions ( e ) and relative quantification ( f ) in EpiWAT, SVF and purified mature adipocyte fraction from EpiWAT of 16-week NCD- or HFD-fed mice ( n = 3-8). g Representative fluorescence images of B2M (green) and DAPI (blue) staining in EpiWAT sections of NCD-fed (upper) and HFD-fed (below) mice. The right panel quantifies the area-normalized B2M MFI (n = 3). Scale bar = 50 μm. Relative B2m mRNA levels ( h ), protein expressions ( i ) and relative quantification ( j ) in 3T3-L1 adipocytes treated with PA at the indicated dose for 24 h ( n = 4-5). The data are representative as the mean ± standard deviation (SD), with “n” representing the number of biological replicates per experimental group. Significant in d , f , and g was calculated using a two-tailed Student’s t test. Significant in h and j was calculated using one-way ANOVA followed by Tukey’s HSD post hoc test for multiple comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001. B2M β2-microglobulin, DEGs differentially expressed genes, EpiWAT epididymal adipose tissue, HFD high-fat diet, MFI mean fluorescence intensity, NCD normal chow diet, PA palmitic acid, SVF stromal vascular fraction
    B2m, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+%CE%B22-microglobulin/%CE%B22+Microglobulin+Antibody%2C+anti-human%2C+PE%2C+REAdye_lease/bio_rxiv__2025__08__06__668737-153-3-37
    Average 94 stars, based on 1 article reviews
    b2m - by Bioz Stars, 2026-10
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    human β2-microglobulin  (Millipore)
    90
    Millipore human β2-microglobulin
    a – c A transcriptome analysis of mature adipocytes isolated from EpiWAT of C57BL/6J mice on 16-week NCD and HFD. a Network diagram illustrating the interaction among the top 10 immune-related pathways enriched by KEGG analysis of DEGs. The table showing the degree of interaction for each pathway, as assessed by the cumulative interaction scores of the genes within the pathway. b GSEA of DEGs showing two <t>B2M-associated</t> pathways in adipocytes. c Network diagram illustrating the interaction among genes in antigen processing and presentation pathway (blue) and iron uptake and transport pathway (green), with B2M (red) at the intersection. Relative B2m mRNA levels ( d ), protein expressions ( e ) and relative quantification ( f ) in EpiWAT, SVF and purified mature adipocyte fraction from EpiWAT of 16-week NCD- or HFD-fed mice ( n = 3-8). g Representative fluorescence images of B2M (green) and DAPI (blue) staining in EpiWAT sections of NCD-fed (upper) and HFD-fed (below) mice. The right panel quantifies the area-normalized B2M MFI (n = 3). Scale bar = 50 μm. Relative B2m mRNA levels ( h ), protein expressions ( i ) and relative quantification ( j ) in 3T3-L1 adipocytes treated with PA at the indicated dose for 24 h ( n = 4-5). The data are representative as the mean ± standard deviation (SD), with “n” representing the number of biological replicates per experimental group. Significant in d , f , and g was calculated using a two-tailed Student’s t test. Significant in h and j was calculated using one-way ANOVA followed by Tukey’s HSD post hoc test for multiple comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001. B2M β2-microglobulin, DEGs differentially expressed genes, EpiWAT epididymal adipose tissue, HFD high-fat diet, MFI mean fluorescence intensity, NCD normal chow diet, PA palmitic acid, SVF stromal vascular fraction
    Human β2 Microglobulin, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+%CE%B22-microglobulin/human+%CE%B22+microglobulin/us12331097-1543-24-26
    Average 90 stars, based on 1 article reviews
    human β2-microglobulin - by Bioz Stars, 2026-10
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    Image Search Results


    a – c A transcriptome analysis of mature adipocytes isolated from EpiWAT of C57BL/6J mice on 16-week NCD and HFD. a Network diagram illustrating the interaction among the top 10 immune-related pathways enriched by KEGG analysis of DEGs. The table showing the degree of interaction for each pathway, as assessed by the cumulative interaction scores of the genes within the pathway. b GSEA of DEGs showing two B2M-associated pathways in adipocytes. c Network diagram illustrating the interaction among genes in antigen processing and presentation pathway (blue) and iron uptake and transport pathway (green), with B2M (red) at the intersection. Relative B2m mRNA levels ( d ), protein expressions ( e ) and relative quantification ( f ) in EpiWAT, SVF and purified mature adipocyte fraction from EpiWAT of 16-week NCD- or HFD-fed mice ( n = 3-8). g Representative fluorescence images of B2M (green) and DAPI (blue) staining in EpiWAT sections of NCD-fed (upper) and HFD-fed (below) mice. The right panel quantifies the area-normalized B2M MFI (n = 3). Scale bar = 50 μm. Relative B2m mRNA levels ( h ), protein expressions ( i ) and relative quantification ( j ) in 3T3-L1 adipocytes treated with PA at the indicated dose for 24 h ( n = 4-5). The data are representative as the mean ± standard deviation (SD), with “n” representing the number of biological replicates per experimental group. Significant in d , f , and g was calculated using a two-tailed Student’s t test. Significant in h and j was calculated using one-way ANOVA followed by Tukey’s HSD post hoc test for multiple comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001. B2M β2-microglobulin, DEGs differentially expressed genes, EpiWAT epididymal adipose tissue, HFD high-fat diet, MFI mean fluorescence intensity, NCD normal chow diet, PA palmitic acid, SVF stromal vascular fraction

    Journal: Signal Transduction and Targeted Therapy

    Article Title: Adipocytes orchestrate obesity-related chronic inflammation through β2-microglobulin

    doi: 10.1038/s41392-025-02486-3

    Figure Lengend Snippet: a – c A transcriptome analysis of mature adipocytes isolated from EpiWAT of C57BL/6J mice on 16-week NCD and HFD. a Network diagram illustrating the interaction among the top 10 immune-related pathways enriched by KEGG analysis of DEGs. The table showing the degree of interaction for each pathway, as assessed by the cumulative interaction scores of the genes within the pathway. b GSEA of DEGs showing two B2M-associated pathways in adipocytes. c Network diagram illustrating the interaction among genes in antigen processing and presentation pathway (blue) and iron uptake and transport pathway (green), with B2M (red) at the intersection. Relative B2m mRNA levels ( d ), protein expressions ( e ) and relative quantification ( f ) in EpiWAT, SVF and purified mature adipocyte fraction from EpiWAT of 16-week NCD- or HFD-fed mice ( n = 3-8). g Representative fluorescence images of B2M (green) and DAPI (blue) staining in EpiWAT sections of NCD-fed (upper) and HFD-fed (below) mice. The right panel quantifies the area-normalized B2M MFI (n = 3). Scale bar = 50 μm. Relative B2m mRNA levels ( h ), protein expressions ( i ) and relative quantification ( j ) in 3T3-L1 adipocytes treated with PA at the indicated dose for 24 h ( n = 4-5). The data are representative as the mean ± standard deviation (SD), with “n” representing the number of biological replicates per experimental group. Significant in d , f , and g was calculated using a two-tailed Student’s t test. Significant in h and j was calculated using one-way ANOVA followed by Tukey’s HSD post hoc test for multiple comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001. B2M β2-microglobulin, DEGs differentially expressed genes, EpiWAT epididymal adipose tissue, HFD high-fat diet, MFI mean fluorescence intensity, NCD normal chow diet, PA palmitic acid, SVF stromal vascular fraction

    Article Snippet: ATMs were isolated from the EpiWAT of NCD-fed B2m f/f mice and sorted with magnetic beads (130-110-443, Miltenyi Biotec).

    Techniques: Isolation, Quantitative Proteomics, Purification, Fluorescence, Staining, Standard Deviation, Two Tailed Test

    a Study design of diet induced obesity model. b , c Representative photograph of B2m f/f and B2m cKO mice and their respective fat pads. d Body weights ( n = 8). e Weight and proportion of EpiWAT ( n = 8). f Body content (fat, lean, water) analyzed by NMR spectrometer ( n = 6–8). Representative histological images showing H&E staining and Oil Red O staining of indicated sections of HFD-fed B2m f/f and B2m cKO mice ( g ), with quantitation of average adipocyte size of EpiWAT and SAT section ( h ), NAS ( i ), and quantitation of Oil Red O staining ( j ) ( n = 3). Scale bars, 100 μm. k – n Serum levels of leptin, adiponectin, insulin, and glucagon in HFD-fed B2m f/f and B2m cKO mice after 4 hours of fasting ( n = 6). o , p IPGTT (2 g glucose per kg body weight), IPITT (0.75 U insulin per kg body weight) and their respective AUC ( n = 8). q – t Serum levels of TG, TC, HDL and LDL ( n = 8). u Serum levels of TNFα, IFNγ, IL-1β, and IL-6 ( n = 7-8). The data are represented as the mean ± standard deviation (SD), with “n” representing the number of biological replicates per experimental group. Significance in e , f , h , j – n , AUC in o , AUC in p , and q – u was calculated using a two-tailed Student’s t test. Significance in d , o , and p was calculated using two-way ANOVA followed by Tukey’s HSD post hoc test for multiple comparisons. Significance in i was calculated using the Mann-Whitney U test. * p < 0.05, ** p < 0.01, *** p < 0.001. AUC area under the curve, BAT brown adipose tissue, EpiWAT epididymal adipose tissue, HDL high density lipoprotein, HFD high-fat diet, IPGTT intraperitoneal glucose tolerance test, IPITT intraperitoneal insulin tolerance test, LDL low density lipoprotein, NAS Non-alcoholic fatty liver disease activity score, NCD normal chow diet, SAT subcutaneous adipose tissue, TC cholesterol, TG triglyceride

    Journal: Signal Transduction and Targeted Therapy

    Article Title: Adipocytes orchestrate obesity-related chronic inflammation through β2-microglobulin

    doi: 10.1038/s41392-025-02486-3

    Figure Lengend Snippet: a Study design of diet induced obesity model. b , c Representative photograph of B2m f/f and B2m cKO mice and their respective fat pads. d Body weights ( n = 8). e Weight and proportion of EpiWAT ( n = 8). f Body content (fat, lean, water) analyzed by NMR spectrometer ( n = 6–8). Representative histological images showing H&E staining and Oil Red O staining of indicated sections of HFD-fed B2m f/f and B2m cKO mice ( g ), with quantitation of average adipocyte size of EpiWAT and SAT section ( h ), NAS ( i ), and quantitation of Oil Red O staining ( j ) ( n = 3). Scale bars, 100 μm. k – n Serum levels of leptin, adiponectin, insulin, and glucagon in HFD-fed B2m f/f and B2m cKO mice after 4 hours of fasting ( n = 6). o , p IPGTT (2 g glucose per kg body weight), IPITT (0.75 U insulin per kg body weight) and their respective AUC ( n = 8). q – t Serum levels of TG, TC, HDL and LDL ( n = 8). u Serum levels of TNFα, IFNγ, IL-1β, and IL-6 ( n = 7-8). The data are represented as the mean ± standard deviation (SD), with “n” representing the number of biological replicates per experimental group. Significance in e , f , h , j – n , AUC in o , AUC in p , and q – u was calculated using a two-tailed Student’s t test. Significance in d , o , and p was calculated using two-way ANOVA followed by Tukey’s HSD post hoc test for multiple comparisons. Significance in i was calculated using the Mann-Whitney U test. * p < 0.05, ** p < 0.01, *** p < 0.001. AUC area under the curve, BAT brown adipose tissue, EpiWAT epididymal adipose tissue, HDL high density lipoprotein, HFD high-fat diet, IPGTT intraperitoneal glucose tolerance test, IPITT intraperitoneal insulin tolerance test, LDL low density lipoprotein, NAS Non-alcoholic fatty liver disease activity score, NCD normal chow diet, SAT subcutaneous adipose tissue, TC cholesterol, TG triglyceride

    Article Snippet: ATMs were isolated from the EpiWAT of NCD-fed B2m f/f mice and sorted with magnetic beads (130-110-443, Miltenyi Biotec).

    Techniques: Staining, Quantitation Assay, Standard Deviation, Two Tailed Test, MANN-WHITNEY, Activity Assay

    a GSEA of antigen processing and presentation pathway between HFD-fed WT mice and B2m cKO mice. Relative mRNA levels of H2-K1 ( b ) and H2-D1 ( c ) in isolated adipocyte fraction of EpiWAT from NCD- or HFD-fed B2m f/f and B2m cKO mice ( n = 4). Protein expressions and relative quantitation of MHC Class I in whole cell lysates ( d , e ) and membrane component ( f , g ) derived from adipocyte fraction isolated from EpiWAT of NCD- or HFD-fed B2m f/f and B2m cKO mice ( n = 3). h Schematic representation of the in vitro co-culture experiment involving primary adipocytes and CD8 + T cells. CD8 + T cells were isolated from EpiWAT of obese B2m f/f mice using a magnetic bead sorting kit and labeled with CFSE before co-culture. Adipocytes were induced from primary adipocyte precursors in SVF and treated with or without PA (2.5 mM) for 24 hours before co-culture. Flow cytometric analysis of proliferation ( i , j ) and CD69 ( k , l ) expression in CD8 + T cells following co-culture with primary adipocytes. ( n = 3). The data are representative as the mean ± standard deviation (SD), with “n” representing the number of biological replicates per experimental group. Significant in b – d , and f was calculated using two-way ANOVA followed by Tukey’s HSD post hoc test for multiple comparisons. Significant in j and l was calculated using multi-way ANOVA followed by Tukey’s HSD post hoc test for multiple comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001. CFSE Carboxyfluorescein Succinimidyl Ester, EpiWAT epididymal adipose tissue, HFD high fat diet, NCD normal chow diet, PA palmitic acid, SVF stromal vascular fraction, WT wild type

    Journal: Signal Transduction and Targeted Therapy

    Article Title: Adipocytes orchestrate obesity-related chronic inflammation through β2-microglobulin

    doi: 10.1038/s41392-025-02486-3

    Figure Lengend Snippet: a GSEA of antigen processing and presentation pathway between HFD-fed WT mice and B2m cKO mice. Relative mRNA levels of H2-K1 ( b ) and H2-D1 ( c ) in isolated adipocyte fraction of EpiWAT from NCD- or HFD-fed B2m f/f and B2m cKO mice ( n = 4). Protein expressions and relative quantitation of MHC Class I in whole cell lysates ( d , e ) and membrane component ( f , g ) derived from adipocyte fraction isolated from EpiWAT of NCD- or HFD-fed B2m f/f and B2m cKO mice ( n = 3). h Schematic representation of the in vitro co-culture experiment involving primary adipocytes and CD8 + T cells. CD8 + T cells were isolated from EpiWAT of obese B2m f/f mice using a magnetic bead sorting kit and labeled with CFSE before co-culture. Adipocytes were induced from primary adipocyte precursors in SVF and treated with or without PA (2.5 mM) for 24 hours before co-culture. Flow cytometric analysis of proliferation ( i , j ) and CD69 ( k , l ) expression in CD8 + T cells following co-culture with primary adipocytes. ( n = 3). The data are representative as the mean ± standard deviation (SD), with “n” representing the number of biological replicates per experimental group. Significant in b – d , and f was calculated using two-way ANOVA followed by Tukey’s HSD post hoc test for multiple comparisons. Significant in j and l was calculated using multi-way ANOVA followed by Tukey’s HSD post hoc test for multiple comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001. CFSE Carboxyfluorescein Succinimidyl Ester, EpiWAT epididymal adipose tissue, HFD high fat diet, NCD normal chow diet, PA palmitic acid, SVF stromal vascular fraction, WT wild type

    Article Snippet: ATMs were isolated from the EpiWAT of NCD-fed B2m f/f mice and sorted with magnetic beads (130-110-443, Miltenyi Biotec).

    Techniques: Isolation, Quantitation Assay, Membrane, Derivative Assay, In Vitro, Co-Culture Assay, Labeling, Expressing, Standard Deviation

    a Relative mRNA levels of Fth and Ftl in the adipocyte fraction from EpiWAT of NCD- or HFD-fed B2m f/f and B2m cKO mice ( n = 8). Protein expressions ( b ) and relative quantitation normalized to the internal reference Actin ( c ) of FTH1 and FTL1 in the lysates of mature adipocytes isolated from EpiWAT of NCD- or HFD-fed B2m f/f and B2m cKO mice ( n = 3). d Total iron content, as measured by iron assay kit, in mature adipocytes from EpiWAT in NCD- or HFD-fed B2m f/f and B2m cKO mice ( n = 8). e The correlation of total iron content in ( d ) with EpiWAT proportion (%) ( n = 32). f Co-immunoprecipitation assay using HFE as bait protein showing the interaction between HFE, TFR1, and TFR2 within the membrane fractions of adipocytes isolated from EpiWAT in NCD- or HFD-fed B2m f/f and B2m cKO mice. Relative mRNA levels of Tfr2 ( g ), Hfe ( h ), Tfr1 ( i ), and HAMP ( j ) in the adipocyte fraction isolated from EpiWAT in NCD- or HFD-fed B2m f/f and B2m cKO mice ( n = 7-8). Protein expressions ( k ) and relative quantitation normalized to the internal reference Vinculin in TFR2 ( l ), HFE ( m ), TFR1 ( n ), hepcidin ( o ), and FPN ( p ) in the lysates of adipocyte fraction isolated from EpiWAT in NCD- or HFD-fed B2m f/f and B2m cKO mice ( n = 3). Protein expressions ( q ) and relative quantitation normalized to the internal reference Na,K-ATPase of TFR2 ( r ), HFE ( s ), TFR1 ( t ), and FPN ( u ) in membrane component derived from adipocyte fraction isolated from EpiWAT in NCD- or HFD-fed B2m f/f and B2m cKO mice ( n = 3). The data are represented as the mean ± standard deviation (SD), with “n” representing the number of biological replicates per experimental group. Significant in a , c , d , g – j , l – p , and r – u was calculated using two-way ANOVA followed by Tukey’s HSD post hoc test for multiple comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001.EpiWAT epididymal adipose tissue, FTH ferritin heavy chain, FTL ferritin light chain, HFD high fat diet, HFE Hereditary hemochromatosis protein, NCD normal chow diet, TFR1 transferrin receptor 1, TFR2 transferrin receptor 2, FPN ferroportin

    Journal: Signal Transduction and Targeted Therapy

    Article Title: Adipocytes orchestrate obesity-related chronic inflammation through β2-microglobulin

    doi: 10.1038/s41392-025-02486-3

    Figure Lengend Snippet: a Relative mRNA levels of Fth and Ftl in the adipocyte fraction from EpiWAT of NCD- or HFD-fed B2m f/f and B2m cKO mice ( n = 8). Protein expressions ( b ) and relative quantitation normalized to the internal reference Actin ( c ) of FTH1 and FTL1 in the lysates of mature adipocytes isolated from EpiWAT of NCD- or HFD-fed B2m f/f and B2m cKO mice ( n = 3). d Total iron content, as measured by iron assay kit, in mature adipocytes from EpiWAT in NCD- or HFD-fed B2m f/f and B2m cKO mice ( n = 8). e The correlation of total iron content in ( d ) with EpiWAT proportion (%) ( n = 32). f Co-immunoprecipitation assay using HFE as bait protein showing the interaction between HFE, TFR1, and TFR2 within the membrane fractions of adipocytes isolated from EpiWAT in NCD- or HFD-fed B2m f/f and B2m cKO mice. Relative mRNA levels of Tfr2 ( g ), Hfe ( h ), Tfr1 ( i ), and HAMP ( j ) in the adipocyte fraction isolated from EpiWAT in NCD- or HFD-fed B2m f/f and B2m cKO mice ( n = 7-8). Protein expressions ( k ) and relative quantitation normalized to the internal reference Vinculin in TFR2 ( l ), HFE ( m ), TFR1 ( n ), hepcidin ( o ), and FPN ( p ) in the lysates of adipocyte fraction isolated from EpiWAT in NCD- or HFD-fed B2m f/f and B2m cKO mice ( n = 3). Protein expressions ( q ) and relative quantitation normalized to the internal reference Na,K-ATPase of TFR2 ( r ), HFE ( s ), TFR1 ( t ), and FPN ( u ) in membrane component derived from adipocyte fraction isolated from EpiWAT in NCD- or HFD-fed B2m f/f and B2m cKO mice ( n = 3). The data are represented as the mean ± standard deviation (SD), with “n” representing the number of biological replicates per experimental group. Significant in a , c , d , g – j , l – p , and r – u was calculated using two-way ANOVA followed by Tukey’s HSD post hoc test for multiple comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001.EpiWAT epididymal adipose tissue, FTH ferritin heavy chain, FTL ferritin light chain, HFD high fat diet, HFE Hereditary hemochromatosis protein, NCD normal chow diet, TFR1 transferrin receptor 1, TFR2 transferrin receptor 2, FPN ferroportin

    Article Snippet: ATMs were isolated from the EpiWAT of NCD-fed B2m f/f mice and sorted with magnetic beads (130-110-443, Miltenyi Biotec).

    Techniques: Quantitation Assay, Isolation, Iron Assay, Co-Immunoprecipitation Assay, Membrane, Derivative Assay, Standard Deviation

    a – g Adipocytes were induced from primary adipocyte precursors in SVF of NCD-fed B2m f/f and B2m cKO mice and treated with or without PA (2.5 mM) for 24 hours. a cell viability ( n = 4). The levels of ROS ( b , c ), lipid peroxides ( d , e ), and Fe 2+ ( f , g ) in adipocytes were assessed using DCFH, C11-BODIPY 581/591 , and FeRhoNox-1 probes, respectively ( n = 3-6). h – m Adipocytes were induced from primary adipocyte precursors in SVF from EpiWAT of NCD-fed B2m f/f and B2m cKO mice and treated with or without PA (2.5 mM) and/or Erastin (2 μM) for 24 hours. The levels of ROS ( h , i ), lipid peroxides ( j , k ), and Fe 2+ ( l , m ) in adipocytes were assessed using DCFH, C11-BODIPY 581/591 , and FeRhoNox-1 probes, respectively ( n = 3-5). n Ferrous ion content in mature adipocytes from NCD- or HFD-fed B2m f/f and B2m cKO mice, measured using an iron content detection kit ( n = 8). o The correlation of ferrous ion content in ( n ) with EpiWAT proportion (%) in NCD- or HFD-fed B2m f/f and B2m cKO mice ( n = 32). Representative images ( p ) and relative quantification ( q ) of ROS, visualized using staining fluorescent probe DHE, in EpiWAT of HFD-fed B2m f/f and B2m cKO mice, DAPI (blue). ( n = 5). Scale bar, 100 μm. Levels of MDA ( r ) and GSH ( s ) in mature adipocytes isolated from EpiWAT in NCD- or HFD-fed B2m f/f and B2m cKO mice ( n = 8). t Representative transmission electron microscopy images of the mitochondria (indicated by red arrow) of adipocytes in EpiWAT of NCD- or HFD-fed B2m f/f and B2m cKO mice. ( n = 5-6). u Flow cytometry analysis of CD86, CD80, and CD11c expressions on ATMs following 24 hours of co-culture with primary adipocytes. ATMs were isolated from EpiWAT of lean B2m f/f mice via F4/80 + magnetic beads. Primary adipocytes were differentiated from adipocyte precursors in SVF from EpiWAT of lean B2m f/f or B2m cKO mice and treated with or without PA (2.5 mM) for 24 hours prior to co-culture ( n = 4). v Representative immunofluorescence staining images of ATMs in EpiWAT of NCD- or HFD-fed B2m f/f and B2m cKO mice, showing perilipin (green), 4-HNE (orange), F4/80 (cyan), CD206 (yellow), CD11c (red), and DAPI (blue). Scale bar, 50 μm. The data are represented as the mean ± standard deviation (SD), with “n” representing the number of biological replicates per experimental group. Significance in q was calculated using a two-tailed Student’s t test. Significant in a , c , e , g , i , k , m , n , r , s , and u was calculated using two-way ANOVA followed by Tukey’s HSD post hoc test for multiple comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001. 4-HNE 4-hydroxynonenal, ATMs adipose tissue macrophages, DHE Dihydroethidium, EpiWAT epididymal adipose tissue, GSH glutathione, HFD high fat diet, MDA malondialdehyde, NCD normal chow diet, PA palmitic acid, ROS reactive oxygen species, SVF stromal vascular fraction

    Journal: Signal Transduction and Targeted Therapy

    Article Title: Adipocytes orchestrate obesity-related chronic inflammation through β2-microglobulin

    doi: 10.1038/s41392-025-02486-3

    Figure Lengend Snippet: a – g Adipocytes were induced from primary adipocyte precursors in SVF of NCD-fed B2m f/f and B2m cKO mice and treated with or without PA (2.5 mM) for 24 hours. a cell viability ( n = 4). The levels of ROS ( b , c ), lipid peroxides ( d , e ), and Fe 2+ ( f , g ) in adipocytes were assessed using DCFH, C11-BODIPY 581/591 , and FeRhoNox-1 probes, respectively ( n = 3-6). h – m Adipocytes were induced from primary adipocyte precursors in SVF from EpiWAT of NCD-fed B2m f/f and B2m cKO mice and treated with or without PA (2.5 mM) and/or Erastin (2 μM) for 24 hours. The levels of ROS ( h , i ), lipid peroxides ( j , k ), and Fe 2+ ( l , m ) in adipocytes were assessed using DCFH, C11-BODIPY 581/591 , and FeRhoNox-1 probes, respectively ( n = 3-5). n Ferrous ion content in mature adipocytes from NCD- or HFD-fed B2m f/f and B2m cKO mice, measured using an iron content detection kit ( n = 8). o The correlation of ferrous ion content in ( n ) with EpiWAT proportion (%) in NCD- or HFD-fed B2m f/f and B2m cKO mice ( n = 32). Representative images ( p ) and relative quantification ( q ) of ROS, visualized using staining fluorescent probe DHE, in EpiWAT of HFD-fed B2m f/f and B2m cKO mice, DAPI (blue). ( n = 5). Scale bar, 100 μm. Levels of MDA ( r ) and GSH ( s ) in mature adipocytes isolated from EpiWAT in NCD- or HFD-fed B2m f/f and B2m cKO mice ( n = 8). t Representative transmission electron microscopy images of the mitochondria (indicated by red arrow) of adipocytes in EpiWAT of NCD- or HFD-fed B2m f/f and B2m cKO mice. ( n = 5-6). u Flow cytometry analysis of CD86, CD80, and CD11c expressions on ATMs following 24 hours of co-culture with primary adipocytes. ATMs were isolated from EpiWAT of lean B2m f/f mice via F4/80 + magnetic beads. Primary adipocytes were differentiated from adipocyte precursors in SVF from EpiWAT of lean B2m f/f or B2m cKO mice and treated with or without PA (2.5 mM) for 24 hours prior to co-culture ( n = 4). v Representative immunofluorescence staining images of ATMs in EpiWAT of NCD- or HFD-fed B2m f/f and B2m cKO mice, showing perilipin (green), 4-HNE (orange), F4/80 (cyan), CD206 (yellow), CD11c (red), and DAPI (blue). Scale bar, 50 μm. The data are represented as the mean ± standard deviation (SD), with “n” representing the number of biological replicates per experimental group. Significance in q was calculated using a two-tailed Student’s t test. Significant in a , c , e , g , i , k , m , n , r , s , and u was calculated using two-way ANOVA followed by Tukey’s HSD post hoc test for multiple comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001. 4-HNE 4-hydroxynonenal, ATMs adipose tissue macrophages, DHE Dihydroethidium, EpiWAT epididymal adipose tissue, GSH glutathione, HFD high fat diet, MDA malondialdehyde, NCD normal chow diet, PA palmitic acid, ROS reactive oxygen species, SVF stromal vascular fraction

    Article Snippet: ATMs were isolated from the EpiWAT of NCD-fed B2m f/f mice and sorted with magnetic beads (130-110-443, Miltenyi Biotec).

    Techniques: Quantitative Proteomics, Staining, Isolation, Transmission Assay, Electron Microscopy, Flow Cytometry, Co-Culture Assay, Magnetic Beads, Immunofluorescence, Standard Deviation, Two Tailed Test

    a Overview for AAV9-mediated knockdown of B2M in EpiWAT. b Experiment scheme of male mice was injected with either AAV9-Fabp5-ZsGreen- B2m- shRNA (AAV9- B2m ) or AAV9-Fabp5-ZsGreen (AAV9-null) following six-week HFD feeding and subsequently maintained on the same diet for an additional 6 weeks. c Ex vivo organ fluorescence imaging of EpiWAT, Heart, Liver, Spleen, Lung and Kidney from AAV9- B2m mice and age-matched sham-operated mice at 2 weeks post-injection. d Fluorescence intensity of tissues in c ( n = 3). e Relative B2m mRNA expression in EpiWAT, Heart, Liver, Spleen, Lung and Kidney ( n = 3). Protein expression ( f ) and relative quantification ( g ) of B2M in adipocytes derived from EpiWAT ( n = 3). h Representative photograph of AAV9-null and AAV9- B2m mice. i Body weights ( n = 6). j Weight and proportion of EpiWAT ( n = 6). Representative histological images showing H&E staining of EpiWAT ( k ), with quantitation of average adipocyte size ( l ) ( n = 3). Scale bar, 100 μm. m – o Representative histological images showing H&E staining and Oil Red O staining of liver sections, with NAS ( n ) and relative quantitation of Oil Red O staining ( o ) ( n = 3). Scale bar, 100 μm. p – s IPGTT (2 g glucose per kg body weight), IPITT (0.75 U insulin per kg body weight) and their respective AUC ( n = 6). t Serum levels of TG, TC, HDL and LDL ( n = 3). Relative mRNA levels of inflammatory cytokines ( u ) and adipocytokines ( v ) in EpiWAT ( n = 3). The data are representative as the mean ± standard deviation (SD), with “n” representing the number of biological replicates per experimental group. Significant in d , e , g , j , l , o , q , and s – v was calculated using a two-tailed Student’s t test. Significant in i, p and r was calculated using two-way ANOVA followed by Tukey’s HSD post hoc test for multiple comparisons. Significant in n was calculated using Mann-Whitney U test. * p < 0.05, ** p < 0.01, *** p < 0.001. AAV9 type 9 adeno-associated virus, AUC area under the curve, EpiWAT epididymal adipose tissue, HDL high density lipoprotein, HFD high-fat diet, IPGTT intraperitoneal glucose tolerance test, IPITT intraperitoneal insulin tolerance test, LDL low density lipoprotein, NAS Non-alcoholic fatty liver disease activity score, Sham sham-operated group, TC cholesterol, TG triglyceride

    Journal: Signal Transduction and Targeted Therapy

    Article Title: Adipocytes orchestrate obesity-related chronic inflammation through β2-microglobulin

    doi: 10.1038/s41392-025-02486-3

    Figure Lengend Snippet: a Overview for AAV9-mediated knockdown of B2M in EpiWAT. b Experiment scheme of male mice was injected with either AAV9-Fabp5-ZsGreen- B2m- shRNA (AAV9- B2m ) or AAV9-Fabp5-ZsGreen (AAV9-null) following six-week HFD feeding and subsequently maintained on the same diet for an additional 6 weeks. c Ex vivo organ fluorescence imaging of EpiWAT, Heart, Liver, Spleen, Lung and Kidney from AAV9- B2m mice and age-matched sham-operated mice at 2 weeks post-injection. d Fluorescence intensity of tissues in c ( n = 3). e Relative B2m mRNA expression in EpiWAT, Heart, Liver, Spleen, Lung and Kidney ( n = 3). Protein expression ( f ) and relative quantification ( g ) of B2M in adipocytes derived from EpiWAT ( n = 3). h Representative photograph of AAV9-null and AAV9- B2m mice. i Body weights ( n = 6). j Weight and proportion of EpiWAT ( n = 6). Representative histological images showing H&E staining of EpiWAT ( k ), with quantitation of average adipocyte size ( l ) ( n = 3). Scale bar, 100 μm. m – o Representative histological images showing H&E staining and Oil Red O staining of liver sections, with NAS ( n ) and relative quantitation of Oil Red O staining ( o ) ( n = 3). Scale bar, 100 μm. p – s IPGTT (2 g glucose per kg body weight), IPITT (0.75 U insulin per kg body weight) and their respective AUC ( n = 6). t Serum levels of TG, TC, HDL and LDL ( n = 3). Relative mRNA levels of inflammatory cytokines ( u ) and adipocytokines ( v ) in EpiWAT ( n = 3). The data are representative as the mean ± standard deviation (SD), with “n” representing the number of biological replicates per experimental group. Significant in d , e , g , j , l , o , q , and s – v was calculated using a two-tailed Student’s t test. Significant in i, p and r was calculated using two-way ANOVA followed by Tukey’s HSD post hoc test for multiple comparisons. Significant in n was calculated using Mann-Whitney U test. * p < 0.05, ** p < 0.01, *** p < 0.001. AAV9 type 9 adeno-associated virus, AUC area under the curve, EpiWAT epididymal adipose tissue, HDL high density lipoprotein, HFD high-fat diet, IPGTT intraperitoneal glucose tolerance test, IPITT intraperitoneal insulin tolerance test, LDL low density lipoprotein, NAS Non-alcoholic fatty liver disease activity score, Sham sham-operated group, TC cholesterol, TG triglyceride

    Article Snippet: ATMs were isolated from the EpiWAT of NCD-fed B2m f/f mice and sorted with magnetic beads (130-110-443, Miltenyi Biotec).

    Techniques: Knockdown, Injection, shRNA, Ex Vivo, Fluorescence, Imaging, Expressing, Quantitative Proteomics, Derivative Assay, Staining, Quantitation Assay, Standard Deviation, Two Tailed Test, MANN-WHITNEY, Virus, Activity Assay

    KEGG bubble diagram ( a ) and GO bar chart ( b ) displaying selected enrichment pathways of DEGs in SAT from lean and metabolically unhealthy obese patients. c Relative expression of B2m , HLA-A , HLA-B , HLA-C , Fth and Ftl gene in SAT across lean patients ( n = 11), metabolically healthy obese patients ( n = 14) and metabolically unhealthy obese patients ( n = 20). d Correlation analysis of indicated genes and the Mantel test analysis of clinical indicators (BMI, HOMA-IR and mean adipocyte size) and indicated genes among lean and obese-unhealthy patients. A color gradient is utilized to denote Person’s correlation with the value reflecting the precise value of this coefficient. The color of the line indicates the correlation direction in the Mantel test (Mantel’s r. sign), with red indicating a positive correlation and blue signifying a negative correlation. The thickness of the lines reflects the strength of the correlation in the Mantel test (Mantel’s r.abs): thin lines for weak (r < 0.1), standard lines for moderate (0.1 < r < 0.3), and thick lines for strong (r >= 0.3) correlations. The type of line denotes the significance of the Mantel test (Mantel’s p), with solid indicating a significant correlation and dashed signifying a non-significant correlation. e GSEA of DEGs showing significant upregulation of three pathways in SAT from lean patients and metabolically unhealthy obese patients. f Relative expression of B2m in VAT. GSE286454 dataset with lean (n = 4) and obese (n = 3) and GSE283367 ) with lean (n = 70) and obese (n = 43). g – j Protein expressions and relative quantification of B2M, MHC Class I, TFR2, TFR1, FPN, HFE, FTH, FTL and hepcidin in whole cell lysates derived from adipocyte fraction isolated from VAT across lean patients and obese patients ( n = 3). The data are representative as the mean ± standard deviation (SD), with “n” representing the number of biological replicates per experimental group. Significant in f , h , and j was calculated using a two-tailed Student’s t test. Significant in c was calculated using one-way ANOVA followed by Tukey’s HSD post hoc test for multiple comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001. B2M β2-microglobulin, BMI body mass index, DEGs differentially expressed genes, FTH ferritin heavy chain, FTL ferritin light chain, HFE Hereditary hemochromatosis protein, HOMA-IR homeostasis model assessment of insulin resistance, SAT subcutaneous adipose tissue, TFR1 transferrin receptor 1, TFR2 transferrin receptor 2, FPN ferroportin, VAT visceral adipose tissue

    Journal: Signal Transduction and Targeted Therapy

    Article Title: Adipocytes orchestrate obesity-related chronic inflammation through β2-microglobulin

    doi: 10.1038/s41392-025-02486-3

    Figure Lengend Snippet: KEGG bubble diagram ( a ) and GO bar chart ( b ) displaying selected enrichment pathways of DEGs in SAT from lean and metabolically unhealthy obese patients. c Relative expression of B2m , HLA-A , HLA-B , HLA-C , Fth and Ftl gene in SAT across lean patients ( n = 11), metabolically healthy obese patients ( n = 14) and metabolically unhealthy obese patients ( n = 20). d Correlation analysis of indicated genes and the Mantel test analysis of clinical indicators (BMI, HOMA-IR and mean adipocyte size) and indicated genes among lean and obese-unhealthy patients. A color gradient is utilized to denote Person’s correlation with the value reflecting the precise value of this coefficient. The color of the line indicates the correlation direction in the Mantel test (Mantel’s r. sign), with red indicating a positive correlation and blue signifying a negative correlation. The thickness of the lines reflects the strength of the correlation in the Mantel test (Mantel’s r.abs): thin lines for weak (r < 0.1), standard lines for moderate (0.1 < r < 0.3), and thick lines for strong (r >= 0.3) correlations. The type of line denotes the significance of the Mantel test (Mantel’s p), with solid indicating a significant correlation and dashed signifying a non-significant correlation. e GSEA of DEGs showing significant upregulation of three pathways in SAT from lean patients and metabolically unhealthy obese patients. f Relative expression of B2m in VAT. GSE286454 dataset with lean (n = 4) and obese (n = 3) and GSE283367 ) with lean (n = 70) and obese (n = 43). g – j Protein expressions and relative quantification of B2M, MHC Class I, TFR2, TFR1, FPN, HFE, FTH, FTL and hepcidin in whole cell lysates derived from adipocyte fraction isolated from VAT across lean patients and obese patients ( n = 3). The data are representative as the mean ± standard deviation (SD), with “n” representing the number of biological replicates per experimental group. Significant in f , h , and j was calculated using a two-tailed Student’s t test. Significant in c was calculated using one-way ANOVA followed by Tukey’s HSD post hoc test for multiple comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001. B2M β2-microglobulin, BMI body mass index, DEGs differentially expressed genes, FTH ferritin heavy chain, FTL ferritin light chain, HFE Hereditary hemochromatosis protein, HOMA-IR homeostasis model assessment of insulin resistance, SAT subcutaneous adipose tissue, TFR1 transferrin receptor 1, TFR2 transferrin receptor 2, FPN ferroportin, VAT visceral adipose tissue

    Article Snippet: ATMs were isolated from the EpiWAT of NCD-fed B2m f/f mice and sorted with magnetic beads (130-110-443, Miltenyi Biotec).

    Techniques: Metabolic Labelling, Expressing, Quantitative Proteomics, Derivative Assay, Isolation, Standard Deviation, Two Tailed Test

    A schematic illustration of the proposed mechanism by which hypertrophic adipocytes induce chronic adipose inflammation and metabolic disorders associated with obesity. Excessive energy stimulates adipocytes to upregulate B2M expression, which not only facilitates the activation and proliferation of CD8 + T cells in adipose tissue through the endogenous antigen presenting pathway, but also promotes iron overload and subsequent ferroptosis of hypertrophic adipocytes via the HFE/B2M-TFR2-Hepcidin-FPN axis, leading to the polarization of ATMs towards M1, thereby accelerating chronic inflammation metabolic disorders. ATMs, adipose tissue macrophages. This figure was created using Adobe Illustrator

    Journal: Signal Transduction and Targeted Therapy

    Article Title: Adipocytes orchestrate obesity-related chronic inflammation through β2-microglobulin

    doi: 10.1038/s41392-025-02486-3

    Figure Lengend Snippet: A schematic illustration of the proposed mechanism by which hypertrophic adipocytes induce chronic adipose inflammation and metabolic disorders associated with obesity. Excessive energy stimulates adipocytes to upregulate B2M expression, which not only facilitates the activation and proliferation of CD8 + T cells in adipose tissue through the endogenous antigen presenting pathway, but also promotes iron overload and subsequent ferroptosis of hypertrophic adipocytes via the HFE/B2M-TFR2-Hepcidin-FPN axis, leading to the polarization of ATMs towards M1, thereby accelerating chronic inflammation metabolic disorders. ATMs, adipose tissue macrophages. This figure was created using Adobe Illustrator

    Article Snippet: ATMs were isolated from the EpiWAT of NCD-fed B2m f/f mice and sorted with magnetic beads (130-110-443, Miltenyi Biotec).

    Techniques: Expressing, Activation Assay

    a – c A transcriptome analysis of mature adipocytes isolated from EpiWAT of C57BL/6J mice on 16-week NCD and HFD. a Network diagram illustrating the interaction among the top 10 immune-related pathways enriched by KEGG analysis of DEGs. The table showing the degree of interaction for each pathway, as assessed by the cumulative interaction scores of the genes within the pathway. b GSEA of DEGs showing two B2M-associated pathways in adipocytes. c Network diagram illustrating the interaction among genes in antigen processing and presentation pathway (blue) and iron uptake and transport pathway (green), with B2M (red) at the intersection. Relative B2m mRNA levels ( d ), protein expressions ( e ) and relative quantification ( f ) in EpiWAT, SVF and purified mature adipocyte fraction from EpiWAT of 16-week NCD- or HFD-fed mice ( n = 3-8). g Representative fluorescence images of B2M (green) and DAPI (blue) staining in EpiWAT sections of NCD-fed (upper) and HFD-fed (below) mice. The right panel quantifies the area-normalized B2M MFI (n = 3). Scale bar = 50 μm. Relative B2m mRNA levels ( h ), protein expressions ( i ) and relative quantification ( j ) in 3T3-L1 adipocytes treated with PA at the indicated dose for 24 h ( n = 4-5). The data are representative as the mean ± standard deviation (SD), with “n” representing the number of biological replicates per experimental group. Significant in d , f , and g was calculated using a two-tailed Student’s t test. Significant in h and j was calculated using one-way ANOVA followed by Tukey’s HSD post hoc test for multiple comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001. B2M β2-microglobulin, DEGs differentially expressed genes, EpiWAT epididymal adipose tissue, HFD high-fat diet, MFI mean fluorescence intensity, NCD normal chow diet, PA palmitic acid, SVF stromal vascular fraction

    Journal: Signal Transduction and Targeted Therapy

    Article Title: Adipocytes orchestrate obesity-related chronic inflammation through β2-microglobulin

    doi: 10.1038/s41392-025-02486-3

    Figure Lengend Snippet: a – c A transcriptome analysis of mature adipocytes isolated from EpiWAT of C57BL/6J mice on 16-week NCD and HFD. a Network diagram illustrating the interaction among the top 10 immune-related pathways enriched by KEGG analysis of DEGs. The table showing the degree of interaction for each pathway, as assessed by the cumulative interaction scores of the genes within the pathway. b GSEA of DEGs showing two B2M-associated pathways in adipocytes. c Network diagram illustrating the interaction among genes in antigen processing and presentation pathway (blue) and iron uptake and transport pathway (green), with B2M (red) at the intersection. Relative B2m mRNA levels ( d ), protein expressions ( e ) and relative quantification ( f ) in EpiWAT, SVF and purified mature adipocyte fraction from EpiWAT of 16-week NCD- or HFD-fed mice ( n = 3-8). g Representative fluorescence images of B2M (green) and DAPI (blue) staining in EpiWAT sections of NCD-fed (upper) and HFD-fed (below) mice. The right panel quantifies the area-normalized B2M MFI (n = 3). Scale bar = 50 μm. Relative B2m mRNA levels ( h ), protein expressions ( i ) and relative quantification ( j ) in 3T3-L1 adipocytes treated with PA at the indicated dose for 24 h ( n = 4-5). The data are representative as the mean ± standard deviation (SD), with “n” representing the number of biological replicates per experimental group. Significant in d , f , and g was calculated using a two-tailed Student’s t test. Significant in h and j was calculated using one-way ANOVA followed by Tukey’s HSD post hoc test for multiple comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001. B2M β2-microglobulin, DEGs differentially expressed genes, EpiWAT epididymal adipose tissue, HFD high-fat diet, MFI mean fluorescence intensity, NCD normal chow diet, PA palmitic acid, SVF stromal vascular fraction

    Article Snippet: CD8 + T cells were isolated from the EpiWAT of HFD-fed B2m f/f mice via magnetic beads (130-104-075, Miltenyi Biotec).

    Techniques: Isolation, Quantitative Proteomics, Purification, Fluorescence, Staining, Standard Deviation, Two Tailed Test

    a Study design of diet induced obesity model. b , c Representative photograph of B2m f/f and B2m cKO mice and their respective fat pads. d Body weights ( n = 8). e Weight and proportion of EpiWAT ( n = 8). f Body content (fat, lean, water) analyzed by NMR spectrometer ( n = 6–8). Representative histological images showing H&E staining and Oil Red O staining of indicated sections of HFD-fed B2m f/f and B2m cKO mice ( g ), with quantitation of average adipocyte size of EpiWAT and SAT section ( h ), NAS ( i ), and quantitation of Oil Red O staining ( j ) ( n = 3). Scale bars, 100 μm. k – n Serum levels of leptin, adiponectin, insulin, and glucagon in HFD-fed B2m f/f and B2m cKO mice after 4 hours of fasting ( n = 6). o , p IPGTT (2 g glucose per kg body weight), IPITT (0.75 U insulin per kg body weight) and their respective AUC ( n = 8). q – t Serum levels of TG, TC, HDL and LDL ( n = 8). u Serum levels of TNFα, IFNγ, IL-1β, and IL-6 ( n = 7-8). The data are represented as the mean ± standard deviation (SD), with “n” representing the number of biological replicates per experimental group. Significance in e , f , h , j – n , AUC in o , AUC in p , and q – u was calculated using a two-tailed Student’s t test. Significance in d , o , and p was calculated using two-way ANOVA followed by Tukey’s HSD post hoc test for multiple comparisons. Significance in i was calculated using the Mann-Whitney U test. * p < 0.05, ** p < 0.01, *** p < 0.001. AUC area under the curve, BAT brown adipose tissue, EpiWAT epididymal adipose tissue, HDL high density lipoprotein, HFD high-fat diet, IPGTT intraperitoneal glucose tolerance test, IPITT intraperitoneal insulin tolerance test, LDL low density lipoprotein, NAS Non-alcoholic fatty liver disease activity score, NCD normal chow diet, SAT subcutaneous adipose tissue, TC cholesterol, TG triglyceride

    Journal: Signal Transduction and Targeted Therapy

    Article Title: Adipocytes orchestrate obesity-related chronic inflammation through β2-microglobulin

    doi: 10.1038/s41392-025-02486-3

    Figure Lengend Snippet: a Study design of diet induced obesity model. b , c Representative photograph of B2m f/f and B2m cKO mice and their respective fat pads. d Body weights ( n = 8). e Weight and proportion of EpiWAT ( n = 8). f Body content (fat, lean, water) analyzed by NMR spectrometer ( n = 6–8). Representative histological images showing H&E staining and Oil Red O staining of indicated sections of HFD-fed B2m f/f and B2m cKO mice ( g ), with quantitation of average adipocyte size of EpiWAT and SAT section ( h ), NAS ( i ), and quantitation of Oil Red O staining ( j ) ( n = 3). Scale bars, 100 μm. k – n Serum levels of leptin, adiponectin, insulin, and glucagon in HFD-fed B2m f/f and B2m cKO mice after 4 hours of fasting ( n = 6). o , p IPGTT (2 g glucose per kg body weight), IPITT (0.75 U insulin per kg body weight) and their respective AUC ( n = 8). q – t Serum levels of TG, TC, HDL and LDL ( n = 8). u Serum levels of TNFα, IFNγ, IL-1β, and IL-6 ( n = 7-8). The data are represented as the mean ± standard deviation (SD), with “n” representing the number of biological replicates per experimental group. Significance in e , f , h , j – n , AUC in o , AUC in p , and q – u was calculated using a two-tailed Student’s t test. Significance in d , o , and p was calculated using two-way ANOVA followed by Tukey’s HSD post hoc test for multiple comparisons. Significance in i was calculated using the Mann-Whitney U test. * p < 0.05, ** p < 0.01, *** p < 0.001. AUC area under the curve, BAT brown adipose tissue, EpiWAT epididymal adipose tissue, HDL high density lipoprotein, HFD high-fat diet, IPGTT intraperitoneal glucose tolerance test, IPITT intraperitoneal insulin tolerance test, LDL low density lipoprotein, NAS Non-alcoholic fatty liver disease activity score, NCD normal chow diet, SAT subcutaneous adipose tissue, TC cholesterol, TG triglyceride

    Article Snippet: CD8 + T cells were isolated from the EpiWAT of HFD-fed B2m f/f mice via magnetic beads (130-104-075, Miltenyi Biotec).

    Techniques: Staining, Quantitation Assay, Standard Deviation, Two Tailed Test, MANN-WHITNEY, Activity Assay

    a GSEA of antigen processing and presentation pathway between HFD-fed WT mice and B2m cKO mice. Relative mRNA levels of H2-K1 ( b ) and H2-D1 ( c ) in isolated adipocyte fraction of EpiWAT from NCD- or HFD-fed B2m f/f and B2m cKO mice ( n = 4). Protein expressions and relative quantitation of MHC Class I in whole cell lysates ( d , e ) and membrane component ( f , g ) derived from adipocyte fraction isolated from EpiWAT of NCD- or HFD-fed B2m f/f and B2m cKO mice ( n = 3). h Schematic representation of the in vitro co-culture experiment involving primary adipocytes and CD8 + T cells. CD8 + T cells were isolated from EpiWAT of obese B2m f/f mice using a magnetic bead sorting kit and labeled with CFSE before co-culture. Adipocytes were induced from primary adipocyte precursors in SVF and treated with or without PA (2.5 mM) for 24 hours before co-culture. Flow cytometric analysis of proliferation ( i , j ) and CD69 ( k , l ) expression in CD8 + T cells following co-culture with primary adipocytes. ( n = 3). The data are representative as the mean ± standard deviation (SD), with “n” representing the number of biological replicates per experimental group. Significant in b – d , and f was calculated using two-way ANOVA followed by Tukey’s HSD post hoc test for multiple comparisons. Significant in j and l was calculated using multi-way ANOVA followed by Tukey’s HSD post hoc test for multiple comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001. CFSE Carboxyfluorescein Succinimidyl Ester, EpiWAT epididymal adipose tissue, HFD high fat diet, NCD normal chow diet, PA palmitic acid, SVF stromal vascular fraction, WT wild type

    Journal: Signal Transduction and Targeted Therapy

    Article Title: Adipocytes orchestrate obesity-related chronic inflammation through β2-microglobulin

    doi: 10.1038/s41392-025-02486-3

    Figure Lengend Snippet: a GSEA of antigen processing and presentation pathway between HFD-fed WT mice and B2m cKO mice. Relative mRNA levels of H2-K1 ( b ) and H2-D1 ( c ) in isolated adipocyte fraction of EpiWAT from NCD- or HFD-fed B2m f/f and B2m cKO mice ( n = 4). Protein expressions and relative quantitation of MHC Class I in whole cell lysates ( d , e ) and membrane component ( f , g ) derived from adipocyte fraction isolated from EpiWAT of NCD- or HFD-fed B2m f/f and B2m cKO mice ( n = 3). h Schematic representation of the in vitro co-culture experiment involving primary adipocytes and CD8 + T cells. CD8 + T cells were isolated from EpiWAT of obese B2m f/f mice using a magnetic bead sorting kit and labeled with CFSE before co-culture. Adipocytes were induced from primary adipocyte precursors in SVF and treated with or without PA (2.5 mM) for 24 hours before co-culture. Flow cytometric analysis of proliferation ( i , j ) and CD69 ( k , l ) expression in CD8 + T cells following co-culture with primary adipocytes. ( n = 3). The data are representative as the mean ± standard deviation (SD), with “n” representing the number of biological replicates per experimental group. Significant in b – d , and f was calculated using two-way ANOVA followed by Tukey’s HSD post hoc test for multiple comparisons. Significant in j and l was calculated using multi-way ANOVA followed by Tukey’s HSD post hoc test for multiple comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001. CFSE Carboxyfluorescein Succinimidyl Ester, EpiWAT epididymal adipose tissue, HFD high fat diet, NCD normal chow diet, PA palmitic acid, SVF stromal vascular fraction, WT wild type

    Article Snippet: CD8 + T cells were isolated from the EpiWAT of HFD-fed B2m f/f mice via magnetic beads (130-104-075, Miltenyi Biotec).

    Techniques: Isolation, Quantitation Assay, Membrane, Derivative Assay, In Vitro, Co-Culture Assay, Labeling, Expressing, Standard Deviation

    a Relative mRNA levels of Fth and Ftl in the adipocyte fraction from EpiWAT of NCD- or HFD-fed B2m f/f and B2m cKO mice ( n = 8). Protein expressions ( b ) and relative quantitation normalized to the internal reference Actin ( c ) of FTH1 and FTL1 in the lysates of mature adipocytes isolated from EpiWAT of NCD- or HFD-fed B2m f/f and B2m cKO mice ( n = 3). d Total iron content, as measured by iron assay kit, in mature adipocytes from EpiWAT in NCD- or HFD-fed B2m f/f and B2m cKO mice ( n = 8). e The correlation of total iron content in ( d ) with EpiWAT proportion (%) ( n = 32). f Co-immunoprecipitation assay using HFE as bait protein showing the interaction between HFE, TFR1, and TFR2 within the membrane fractions of adipocytes isolated from EpiWAT in NCD- or HFD-fed B2m f/f and B2m cKO mice. Relative mRNA levels of Tfr2 ( g ), Hfe ( h ), Tfr1 ( i ), and HAMP ( j ) in the adipocyte fraction isolated from EpiWAT in NCD- or HFD-fed B2m f/f and B2m cKO mice ( n = 7-8). Protein expressions ( k ) and relative quantitation normalized to the internal reference Vinculin in TFR2 ( l ), HFE ( m ), TFR1 ( n ), hepcidin ( o ), and FPN ( p ) in the lysates of adipocyte fraction isolated from EpiWAT in NCD- or HFD-fed B2m f/f and B2m cKO mice ( n = 3). Protein expressions ( q ) and relative quantitation normalized to the internal reference Na,K-ATPase of TFR2 ( r ), HFE ( s ), TFR1 ( t ), and FPN ( u ) in membrane component derived from adipocyte fraction isolated from EpiWAT in NCD- or HFD-fed B2m f/f and B2m cKO mice ( n = 3). The data are represented as the mean ± standard deviation (SD), with “n” representing the number of biological replicates per experimental group. Significant in a , c , d , g – j , l – p , and r – u was calculated using two-way ANOVA followed by Tukey’s HSD post hoc test for multiple comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001.EpiWAT epididymal adipose tissue, FTH ferritin heavy chain, FTL ferritin light chain, HFD high fat diet, HFE Hereditary hemochromatosis protein, NCD normal chow diet, TFR1 transferrin receptor 1, TFR2 transferrin receptor 2, FPN ferroportin

    Journal: Signal Transduction and Targeted Therapy

    Article Title: Adipocytes orchestrate obesity-related chronic inflammation through β2-microglobulin

    doi: 10.1038/s41392-025-02486-3

    Figure Lengend Snippet: a Relative mRNA levels of Fth and Ftl in the adipocyte fraction from EpiWAT of NCD- or HFD-fed B2m f/f and B2m cKO mice ( n = 8). Protein expressions ( b ) and relative quantitation normalized to the internal reference Actin ( c ) of FTH1 and FTL1 in the lysates of mature adipocytes isolated from EpiWAT of NCD- or HFD-fed B2m f/f and B2m cKO mice ( n = 3). d Total iron content, as measured by iron assay kit, in mature adipocytes from EpiWAT in NCD- or HFD-fed B2m f/f and B2m cKO mice ( n = 8). e The correlation of total iron content in ( d ) with EpiWAT proportion (%) ( n = 32). f Co-immunoprecipitation assay using HFE as bait protein showing the interaction between HFE, TFR1, and TFR2 within the membrane fractions of adipocytes isolated from EpiWAT in NCD- or HFD-fed B2m f/f and B2m cKO mice. Relative mRNA levels of Tfr2 ( g ), Hfe ( h ), Tfr1 ( i ), and HAMP ( j ) in the adipocyte fraction isolated from EpiWAT in NCD- or HFD-fed B2m f/f and B2m cKO mice ( n = 7-8). Protein expressions ( k ) and relative quantitation normalized to the internal reference Vinculin in TFR2 ( l ), HFE ( m ), TFR1 ( n ), hepcidin ( o ), and FPN ( p ) in the lysates of adipocyte fraction isolated from EpiWAT in NCD- or HFD-fed B2m f/f and B2m cKO mice ( n = 3). Protein expressions ( q ) and relative quantitation normalized to the internal reference Na,K-ATPase of TFR2 ( r ), HFE ( s ), TFR1 ( t ), and FPN ( u ) in membrane component derived from adipocyte fraction isolated from EpiWAT in NCD- or HFD-fed B2m f/f and B2m cKO mice ( n = 3). The data are represented as the mean ± standard deviation (SD), with “n” representing the number of biological replicates per experimental group. Significant in a , c , d , g – j , l – p , and r – u was calculated using two-way ANOVA followed by Tukey’s HSD post hoc test for multiple comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001.EpiWAT epididymal adipose tissue, FTH ferritin heavy chain, FTL ferritin light chain, HFD high fat diet, HFE Hereditary hemochromatosis protein, NCD normal chow diet, TFR1 transferrin receptor 1, TFR2 transferrin receptor 2, FPN ferroportin

    Article Snippet: CD8 + T cells were isolated from the EpiWAT of HFD-fed B2m f/f mice via magnetic beads (130-104-075, Miltenyi Biotec).

    Techniques: Quantitation Assay, Isolation, Iron Assay, Co-Immunoprecipitation Assay, Membrane, Derivative Assay, Standard Deviation

    a – g Adipocytes were induced from primary adipocyte precursors in SVF of NCD-fed B2m f/f and B2m cKO mice and treated with or without PA (2.5 mM) for 24 hours. a cell viability ( n = 4). The levels of ROS ( b , c ), lipid peroxides ( d , e ), and Fe 2+ ( f , g ) in adipocytes were assessed using DCFH, C11-BODIPY 581/591 , and FeRhoNox-1 probes, respectively ( n = 3-6). h – m Adipocytes were induced from primary adipocyte precursors in SVF from EpiWAT of NCD-fed B2m f/f and B2m cKO mice and treated with or without PA (2.5 mM) and/or Erastin (2 μM) for 24 hours. The levels of ROS ( h , i ), lipid peroxides ( j , k ), and Fe 2+ ( l , m ) in adipocytes were assessed using DCFH, C11-BODIPY 581/591 , and FeRhoNox-1 probes, respectively ( n = 3-5). n Ferrous ion content in mature adipocytes from NCD- or HFD-fed B2m f/f and B2m cKO mice, measured using an iron content detection kit ( n = 8). o The correlation of ferrous ion content in ( n ) with EpiWAT proportion (%) in NCD- or HFD-fed B2m f/f and B2m cKO mice ( n = 32). Representative images ( p ) and relative quantification ( q ) of ROS, visualized using staining fluorescent probe DHE, in EpiWAT of HFD-fed B2m f/f and B2m cKO mice, DAPI (blue). ( n = 5). Scale bar, 100 μm. Levels of MDA ( r ) and GSH ( s ) in mature adipocytes isolated from EpiWAT in NCD- or HFD-fed B2m f/f and B2m cKO mice ( n = 8). t Representative transmission electron microscopy images of the mitochondria (indicated by red arrow) of adipocytes in EpiWAT of NCD- or HFD-fed B2m f/f and B2m cKO mice. ( n = 5-6). u Flow cytometry analysis of CD86, CD80, and CD11c expressions on ATMs following 24 hours of co-culture with primary adipocytes. ATMs were isolated from EpiWAT of lean B2m f/f mice via F4/80 + magnetic beads. Primary adipocytes were differentiated from adipocyte precursors in SVF from EpiWAT of lean B2m f/f or B2m cKO mice and treated with or without PA (2.5 mM) for 24 hours prior to co-culture ( n = 4). v Representative immunofluorescence staining images of ATMs in EpiWAT of NCD- or HFD-fed B2m f/f and B2m cKO mice, showing perilipin (green), 4-HNE (orange), F4/80 (cyan), CD206 (yellow), CD11c (red), and DAPI (blue). Scale bar, 50 μm. The data are represented as the mean ± standard deviation (SD), with “n” representing the number of biological replicates per experimental group. Significance in q was calculated using a two-tailed Student’s t test. Significant in a , c , e , g , i , k , m , n , r , s , and u was calculated using two-way ANOVA followed by Tukey’s HSD post hoc test for multiple comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001. 4-HNE 4-hydroxynonenal, ATMs adipose tissue macrophages, DHE Dihydroethidium, EpiWAT epididymal adipose tissue, GSH glutathione, HFD high fat diet, MDA malondialdehyde, NCD normal chow diet, PA palmitic acid, ROS reactive oxygen species, SVF stromal vascular fraction

    Journal: Signal Transduction and Targeted Therapy

    Article Title: Adipocytes orchestrate obesity-related chronic inflammation through β2-microglobulin

    doi: 10.1038/s41392-025-02486-3

    Figure Lengend Snippet: a – g Adipocytes were induced from primary adipocyte precursors in SVF of NCD-fed B2m f/f and B2m cKO mice and treated with or without PA (2.5 mM) for 24 hours. a cell viability ( n = 4). The levels of ROS ( b , c ), lipid peroxides ( d , e ), and Fe 2+ ( f , g ) in adipocytes were assessed using DCFH, C11-BODIPY 581/591 , and FeRhoNox-1 probes, respectively ( n = 3-6). h – m Adipocytes were induced from primary adipocyte precursors in SVF from EpiWAT of NCD-fed B2m f/f and B2m cKO mice and treated with or without PA (2.5 mM) and/or Erastin (2 μM) for 24 hours. The levels of ROS ( h , i ), lipid peroxides ( j , k ), and Fe 2+ ( l , m ) in adipocytes were assessed using DCFH, C11-BODIPY 581/591 , and FeRhoNox-1 probes, respectively ( n = 3-5). n Ferrous ion content in mature adipocytes from NCD- or HFD-fed B2m f/f and B2m cKO mice, measured using an iron content detection kit ( n = 8). o The correlation of ferrous ion content in ( n ) with EpiWAT proportion (%) in NCD- or HFD-fed B2m f/f and B2m cKO mice ( n = 32). Representative images ( p ) and relative quantification ( q ) of ROS, visualized using staining fluorescent probe DHE, in EpiWAT of HFD-fed B2m f/f and B2m cKO mice, DAPI (blue). ( n = 5). Scale bar, 100 μm. Levels of MDA ( r ) and GSH ( s ) in mature adipocytes isolated from EpiWAT in NCD- or HFD-fed B2m f/f and B2m cKO mice ( n = 8). t Representative transmission electron microscopy images of the mitochondria (indicated by red arrow) of adipocytes in EpiWAT of NCD- or HFD-fed B2m f/f and B2m cKO mice. ( n = 5-6). u Flow cytometry analysis of CD86, CD80, and CD11c expressions on ATMs following 24 hours of co-culture with primary adipocytes. ATMs were isolated from EpiWAT of lean B2m f/f mice via F4/80 + magnetic beads. Primary adipocytes were differentiated from adipocyte precursors in SVF from EpiWAT of lean B2m f/f or B2m cKO mice and treated with or without PA (2.5 mM) for 24 hours prior to co-culture ( n = 4). v Representative immunofluorescence staining images of ATMs in EpiWAT of NCD- or HFD-fed B2m f/f and B2m cKO mice, showing perilipin (green), 4-HNE (orange), F4/80 (cyan), CD206 (yellow), CD11c (red), and DAPI (blue). Scale bar, 50 μm. The data are represented as the mean ± standard deviation (SD), with “n” representing the number of biological replicates per experimental group. Significance in q was calculated using a two-tailed Student’s t test. Significant in a , c , e , g , i , k , m , n , r , s , and u was calculated using two-way ANOVA followed by Tukey’s HSD post hoc test for multiple comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001. 4-HNE 4-hydroxynonenal, ATMs adipose tissue macrophages, DHE Dihydroethidium, EpiWAT epididymal adipose tissue, GSH glutathione, HFD high fat diet, MDA malondialdehyde, NCD normal chow diet, PA palmitic acid, ROS reactive oxygen species, SVF stromal vascular fraction

    Article Snippet: CD8 + T cells were isolated from the EpiWAT of HFD-fed B2m f/f mice via magnetic beads (130-104-075, Miltenyi Biotec).

    Techniques: Quantitative Proteomics, Staining, Isolation, Transmission Assay, Electron Microscopy, Flow Cytometry, Co-Culture Assay, Magnetic Beads, Immunofluorescence, Standard Deviation, Two Tailed Test

    a Overview for AAV9-mediated knockdown of B2M in EpiWAT. b Experiment scheme of male mice was injected with either AAV9-Fabp5-ZsGreen- B2m- shRNA (AAV9- B2m ) or AAV9-Fabp5-ZsGreen (AAV9-null) following six-week HFD feeding and subsequently maintained on the same diet for an additional 6 weeks. c Ex vivo organ fluorescence imaging of EpiWAT, Heart, Liver, Spleen, Lung and Kidney from AAV9- B2m mice and age-matched sham-operated mice at 2 weeks post-injection. d Fluorescence intensity of tissues in c ( n = 3). e Relative B2m mRNA expression in EpiWAT, Heart, Liver, Spleen, Lung and Kidney ( n = 3). Protein expression ( f ) and relative quantification ( g ) of B2M in adipocytes derived from EpiWAT ( n = 3). h Representative photograph of AAV9-null and AAV9- B2m mice. i Body weights ( n = 6). j Weight and proportion of EpiWAT ( n = 6). Representative histological images showing H&E staining of EpiWAT ( k ), with quantitation of average adipocyte size ( l ) ( n = 3). Scale bar, 100 μm. m – o Representative histological images showing H&E staining and Oil Red O staining of liver sections, with NAS ( n ) and relative quantitation of Oil Red O staining ( o ) ( n = 3). Scale bar, 100 μm. p – s IPGTT (2 g glucose per kg body weight), IPITT (0.75 U insulin per kg body weight) and their respective AUC ( n = 6). t Serum levels of TG, TC, HDL and LDL ( n = 3). Relative mRNA levels of inflammatory cytokines ( u ) and adipocytokines ( v ) in EpiWAT ( n = 3). The data are representative as the mean ± standard deviation (SD), with “n” representing the number of biological replicates per experimental group. Significant in d , e , g , j , l , o , q , and s – v was calculated using a two-tailed Student’s t test. Significant in i, p and r was calculated using two-way ANOVA followed by Tukey’s HSD post hoc test for multiple comparisons. Significant in n was calculated using Mann-Whitney U test. * p < 0.05, ** p < 0.01, *** p < 0.001. AAV9 type 9 adeno-associated virus, AUC area under the curve, EpiWAT epididymal adipose tissue, HDL high density lipoprotein, HFD high-fat diet, IPGTT intraperitoneal glucose tolerance test, IPITT intraperitoneal insulin tolerance test, LDL low density lipoprotein, NAS Non-alcoholic fatty liver disease activity score, Sham sham-operated group, TC cholesterol, TG triglyceride

    Journal: Signal Transduction and Targeted Therapy

    Article Title: Adipocytes orchestrate obesity-related chronic inflammation through β2-microglobulin

    doi: 10.1038/s41392-025-02486-3

    Figure Lengend Snippet: a Overview for AAV9-mediated knockdown of B2M in EpiWAT. b Experiment scheme of male mice was injected with either AAV9-Fabp5-ZsGreen- B2m- shRNA (AAV9- B2m ) or AAV9-Fabp5-ZsGreen (AAV9-null) following six-week HFD feeding and subsequently maintained on the same diet for an additional 6 weeks. c Ex vivo organ fluorescence imaging of EpiWAT, Heart, Liver, Spleen, Lung and Kidney from AAV9- B2m mice and age-matched sham-operated mice at 2 weeks post-injection. d Fluorescence intensity of tissues in c ( n = 3). e Relative B2m mRNA expression in EpiWAT, Heart, Liver, Spleen, Lung and Kidney ( n = 3). Protein expression ( f ) and relative quantification ( g ) of B2M in adipocytes derived from EpiWAT ( n = 3). h Representative photograph of AAV9-null and AAV9- B2m mice. i Body weights ( n = 6). j Weight and proportion of EpiWAT ( n = 6). Representative histological images showing H&E staining of EpiWAT ( k ), with quantitation of average adipocyte size ( l ) ( n = 3). Scale bar, 100 μm. m – o Representative histological images showing H&E staining and Oil Red O staining of liver sections, with NAS ( n ) and relative quantitation of Oil Red O staining ( o ) ( n = 3). Scale bar, 100 μm. p – s IPGTT (2 g glucose per kg body weight), IPITT (0.75 U insulin per kg body weight) and their respective AUC ( n = 6). t Serum levels of TG, TC, HDL and LDL ( n = 3). Relative mRNA levels of inflammatory cytokines ( u ) and adipocytokines ( v ) in EpiWAT ( n = 3). The data are representative as the mean ± standard deviation (SD), with “n” representing the number of biological replicates per experimental group. Significant in d , e , g , j , l , o , q , and s – v was calculated using a two-tailed Student’s t test. Significant in i, p and r was calculated using two-way ANOVA followed by Tukey’s HSD post hoc test for multiple comparisons. Significant in n was calculated using Mann-Whitney U test. * p < 0.05, ** p < 0.01, *** p < 0.001. AAV9 type 9 adeno-associated virus, AUC area under the curve, EpiWAT epididymal adipose tissue, HDL high density lipoprotein, HFD high-fat diet, IPGTT intraperitoneal glucose tolerance test, IPITT intraperitoneal insulin tolerance test, LDL low density lipoprotein, NAS Non-alcoholic fatty liver disease activity score, Sham sham-operated group, TC cholesterol, TG triglyceride

    Article Snippet: CD8 + T cells were isolated from the EpiWAT of HFD-fed B2m f/f mice via magnetic beads (130-104-075, Miltenyi Biotec).

    Techniques: Knockdown, Injection, shRNA, Ex Vivo, Fluorescence, Imaging, Expressing, Quantitative Proteomics, Derivative Assay, Staining, Quantitation Assay, Standard Deviation, Two Tailed Test, MANN-WHITNEY, Virus, Activity Assay

    KEGG bubble diagram ( a ) and GO bar chart ( b ) displaying selected enrichment pathways of DEGs in SAT from lean and metabolically unhealthy obese patients. c Relative expression of B2m , HLA-A , HLA-B , HLA-C , Fth and Ftl gene in SAT across lean patients ( n = 11), metabolically healthy obese patients ( n = 14) and metabolically unhealthy obese patients ( n = 20). d Correlation analysis of indicated genes and the Mantel test analysis of clinical indicators (BMI, HOMA-IR and mean adipocyte size) and indicated genes among lean and obese-unhealthy patients. A color gradient is utilized to denote Person’s correlation with the value reflecting the precise value of this coefficient. The color of the line indicates the correlation direction in the Mantel test (Mantel’s r. sign), with red indicating a positive correlation and blue signifying a negative correlation. The thickness of the lines reflects the strength of the correlation in the Mantel test (Mantel’s r.abs): thin lines for weak (r < 0.1), standard lines for moderate (0.1 < r < 0.3), and thick lines for strong (r >= 0.3) correlations. The type of line denotes the significance of the Mantel test (Mantel’s p), with solid indicating a significant correlation and dashed signifying a non-significant correlation. e GSEA of DEGs showing significant upregulation of three pathways in SAT from lean patients and metabolically unhealthy obese patients. f Relative expression of B2m in VAT. GSE286454 dataset with lean (n = 4) and obese (n = 3) and GSE283367 ) with lean (n = 70) and obese (n = 43). g – j Protein expressions and relative quantification of B2M, MHC Class I, TFR2, TFR1, FPN, HFE, FTH, FTL and hepcidin in whole cell lysates derived from adipocyte fraction isolated from VAT across lean patients and obese patients ( n = 3). The data are representative as the mean ± standard deviation (SD), with “n” representing the number of biological replicates per experimental group. Significant in f , h , and j was calculated using a two-tailed Student’s t test. Significant in c was calculated using one-way ANOVA followed by Tukey’s HSD post hoc test for multiple comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001. B2M β2-microglobulin, BMI body mass index, DEGs differentially expressed genes, FTH ferritin heavy chain, FTL ferritin light chain, HFE Hereditary hemochromatosis protein, HOMA-IR homeostasis model assessment of insulin resistance, SAT subcutaneous adipose tissue, TFR1 transferrin receptor 1, TFR2 transferrin receptor 2, FPN ferroportin, VAT visceral adipose tissue

    Journal: Signal Transduction and Targeted Therapy

    Article Title: Adipocytes orchestrate obesity-related chronic inflammation through β2-microglobulin

    doi: 10.1038/s41392-025-02486-3

    Figure Lengend Snippet: KEGG bubble diagram ( a ) and GO bar chart ( b ) displaying selected enrichment pathways of DEGs in SAT from lean and metabolically unhealthy obese patients. c Relative expression of B2m , HLA-A , HLA-B , HLA-C , Fth and Ftl gene in SAT across lean patients ( n = 11), metabolically healthy obese patients ( n = 14) and metabolically unhealthy obese patients ( n = 20). d Correlation analysis of indicated genes and the Mantel test analysis of clinical indicators (BMI, HOMA-IR and mean adipocyte size) and indicated genes among lean and obese-unhealthy patients. A color gradient is utilized to denote Person’s correlation with the value reflecting the precise value of this coefficient. The color of the line indicates the correlation direction in the Mantel test (Mantel’s r. sign), with red indicating a positive correlation and blue signifying a negative correlation. The thickness of the lines reflects the strength of the correlation in the Mantel test (Mantel’s r.abs): thin lines for weak (r < 0.1), standard lines for moderate (0.1 < r < 0.3), and thick lines for strong (r >= 0.3) correlations. The type of line denotes the significance of the Mantel test (Mantel’s p), with solid indicating a significant correlation and dashed signifying a non-significant correlation. e GSEA of DEGs showing significant upregulation of three pathways in SAT from lean patients and metabolically unhealthy obese patients. f Relative expression of B2m in VAT. GSE286454 dataset with lean (n = 4) and obese (n = 3) and GSE283367 ) with lean (n = 70) and obese (n = 43). g – j Protein expressions and relative quantification of B2M, MHC Class I, TFR2, TFR1, FPN, HFE, FTH, FTL and hepcidin in whole cell lysates derived from adipocyte fraction isolated from VAT across lean patients and obese patients ( n = 3). The data are representative as the mean ± standard deviation (SD), with “n” representing the number of biological replicates per experimental group. Significant in f , h , and j was calculated using a two-tailed Student’s t test. Significant in c was calculated using one-way ANOVA followed by Tukey’s HSD post hoc test for multiple comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001. B2M β2-microglobulin, BMI body mass index, DEGs differentially expressed genes, FTH ferritin heavy chain, FTL ferritin light chain, HFE Hereditary hemochromatosis protein, HOMA-IR homeostasis model assessment of insulin resistance, SAT subcutaneous adipose tissue, TFR1 transferrin receptor 1, TFR2 transferrin receptor 2, FPN ferroportin, VAT visceral adipose tissue

    Article Snippet: CD8 + T cells were isolated from the EpiWAT of HFD-fed B2m f/f mice via magnetic beads (130-104-075, Miltenyi Biotec).

    Techniques: Metabolic Labelling, Expressing, Quantitative Proteomics, Derivative Assay, Isolation, Standard Deviation, Two Tailed Test

    A schematic illustration of the proposed mechanism by which hypertrophic adipocytes induce chronic adipose inflammation and metabolic disorders associated with obesity. Excessive energy stimulates adipocytes to upregulate B2M expression, which not only facilitates the activation and proliferation of CD8 + T cells in adipose tissue through the endogenous antigen presenting pathway, but also promotes iron overload and subsequent ferroptosis of hypertrophic adipocytes via the HFE/B2M-TFR2-Hepcidin-FPN axis, leading to the polarization of ATMs towards M1, thereby accelerating chronic inflammation metabolic disorders. ATMs, adipose tissue macrophages. This figure was created using Adobe Illustrator

    Journal: Signal Transduction and Targeted Therapy

    Article Title: Adipocytes orchestrate obesity-related chronic inflammation through β2-microglobulin

    doi: 10.1038/s41392-025-02486-3

    Figure Lengend Snippet: A schematic illustration of the proposed mechanism by which hypertrophic adipocytes induce chronic adipose inflammation and metabolic disorders associated with obesity. Excessive energy stimulates adipocytes to upregulate B2M expression, which not only facilitates the activation and proliferation of CD8 + T cells in adipose tissue through the endogenous antigen presenting pathway, but also promotes iron overload and subsequent ferroptosis of hypertrophic adipocytes via the HFE/B2M-TFR2-Hepcidin-FPN axis, leading to the polarization of ATMs towards M1, thereby accelerating chronic inflammation metabolic disorders. ATMs, adipose tissue macrophages. This figure was created using Adobe Illustrator

    Article Snippet: CD8 + T cells were isolated from the EpiWAT of HFD-fed B2m f/f mice via magnetic beads (130-104-075, Miltenyi Biotec).

    Techniques: Expressing, Activation Assay